PMID: 2104942Feb 1, 1990Paper

Point mutations in the Moloney murine leukemia virus enhancer identify a lymphoid-specific viral core motif and 1,3-phorbol myristate acetate-inducible element

Journal of Virology
N A SpeckN Hopkins

Abstract

The transcriptional enhancer of the Moloney murine leukemia virus (MoMLV) is organized as a 75-base-pair repeat, and in each copy of the repeat there are multiple binding sites for nuclear factors. We have introduced point mutations into each of the known nuclear factor-binding sites in the MoMLV enhancer, in both copies of the direct repeat, and have analyzed the transcriptional activity conferred by the mutated enhancers by transient-expression assays in both hematopoietic and nonhematopoietic cell lines. Mutation of individual binding sites in the MoMLV enhancer has moderate effects (less than 2-fold to 20-fold) on transcription in six independent cell lines. Several mutations decreased transcription from the MoMLV enhancer ubiquitously (the leukemia virus factor b site and the glucocorticoid response element), whereas others affected transcription specifically in lymphoid cell lines (core motif) or, more significantly, in fibroblasts (nuclear factor 1 site). The transcriptional activity of the MoMLV enhancer can be induced 8- to 10-fold by 1,3-phorbol myristate acetate in Jurkat T cells. Mutations in any of three adjacent binding sites (leukemia virus factor b and c sites and the core motif) within a 28-base-pair region in ...Continue Reading

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Citations

May 21, 2010·Journal of Virology·James W BrucePaul Ahlquist
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·F U ReussR Heber
Jan 1, 1993·DNA Sequence : the Journal of DNA Sequencing and Mapping·M S RossiJ Zorzópulos
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·G K FuD M Markovitz
Nov 4, 2000·Biology of Reproduction·E S LymarM D Griswold
Feb 27, 1999·Experimental Cell Research·I N MelnikovaB A Christy

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