Point-of-care autofluorescence imaging for real-time sampling and treatment guidance of bioburden in chronic wounds: first-in-human results

PloS One
Ralph S DaCostaRon Linden

Abstract

Traditionally, chronic wound infection is diagnosed by visual inspection under white light and microbiological sampling, which are subjective and suboptimal, respectively, thereby delaying diagnosis and treatment. To address this, we developed a novel handheld, fluorescence imaging device (PRODIGI) that enables non-contact, real-time, high-resolution visualization and differentiation of key pathogenic bacteria through their endogenous autofluorescence, as well as connective tissues in wounds. This was a two-part Phase I, single center, non-randomized trial of chronic wound patients (male and female, ≥18 years; UHN REB #09-0015-A for part 1; UHN REB #12-5003 for part 2; clinicaltrials.gov Identifier: NCT01378728 for part 1 and NCT01651845 for part 2). Part 1 (28 patients; 54% diabetic foot ulcers, 46% non-diabetic wounds) established the feasibility of autofluorescence imaging to accurately guide wound sampling, validated against blinded, gold standard swab-based microbiology. Part 2 (12 patients; 83.3% diabetic foot ulcers, 16.7% non-diabetic wounds) established the feasibility of autofluorescence imaging to guide wound treatment and quantitatively assess treatment response. We showed that PRODIGI can be used to guide and impro...Continue Reading

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Citations

Sep 8, 2016·Advances in Wound Care·Maanasa JayachandranAnuradha Godavarty
Sep 8, 2016·Advances in Wound Care·Jeffrey E ThatcherJ Michael DiMaio
Mar 8, 2018·Journal of Biomedical Optics·Brian C WilsonFrederic Leblond
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Jun 16, 2021·Advances in Wound Care·Maximillian A WeigeltIra M Herman
Nov 12, 2020·ACS Sensors·Olja SimoskaKeith J Stevenson

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Methods Mentioned

BETA
biopsy
biopsies
fluorescence imaging
lavage

Clinical Trials Mentioned

NCT01378728
NCT01651845

Software Mentioned

Pressure Sore Status Tool ( PSST )
MATLAB
R
PRODIGI
SAS

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