Point-of-care diagnostic assay for the detection of Zika virus using the recombinase polymerase amplification method

The Journal of General Virology
Nadina I Vasileva WandRoger Hewson

Abstract

The sudden and explosive expansion of Zika virus (ZIKV) from the African continent through Oceania and culminating in the outbreak in South America has highlighted the importance of new rapid point-of-care diagnostic tools for the control and prevention of transmission. ZIKV infection has devastating consequences, such as neurological congenital malformations in infants born to infected mothers and Guillain-Barré syndrome in adults. Additionally, its potential for transmission through vector bites, as well as from person to person through blood transfusions and sexual contact, are important considerations for prompt diagnosis. Recombinase polymerase amplification (RPA), an isothermal method, was developed as an alternative field-applicable assay to PCR. Here we report the development of a novel ZIKV real-time reverse transcriptase RPA (RT-RPA) assay capable of detecting a range of different ZIKV strains from a variety of geographical locations. The ZIKV RT-RPA was shown to be highly sensitive, being capable of detecting as few as five copies of target nucleic acid per reaction, and suitable for use with a battery-operated portable device. The ZIKV RT-RPA demonstrated 100 % specificity and 83 % sensitivity in clinical samples. F...Continue Reading

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Citations

Jun 10, 2019·Molecular Ecology Resources·Molly-Ann WilliamsAnne Parle-McDermott
Aug 1, 2020·PLoS Neglected Tropical Diseases·Laura C BonneyRoger Hewson

Datasets Mentioned

BETA
EU545988
KJ776791
KU321639
BeH819015
KU365778
KU365780
PRVABC59
KU501215
KY288905
KM204119

Methods Mentioned

BETA
enzyme-linked immunosorbent assays
nucleic acid amplification
PCR
Assay

Related Concepts

Zika virus (organism)
Metazoa
Culicidae
RNA, Viral
Statistical Sensitivity
Variation (Genetics)
Reproducibility of Results
Conserved Sequence
Point-of-Care Systems
Nucleic Acid Amplification Tests

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