Point-of-care seeding of nitinol stents with blood-derived endothelial cells

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Alexandra E JantzenHardean E Achneck

Abstract

Nitinol-based vascular devices, for example, peripheral and intracranial stents, are limited by thrombosis and restenosis. To ameliorate these complications, we developed a technology to promote vessel healing by rapidly seeding (QuickSeeding) autologous blood-derived endothelial cells (ECs) onto modified self-expanding nitinol stent delivery systems immediately before implantation. Several thousand micropores were laser-drilled into a delivery system sheath surrounding a commercial nitinol stent to allow for exit of an infused cell suspension. As suspension medium flowed outward through the micropores, ECs flowed through the delivery system attaching to the stent surface. The QuickSeeded ECs adhered to and spread on the stent surface following 24-h in vitro culture under static or flow conditions. Further, QuickSeeded ECs on stents that were deployed into porcine carotid arteries spread to endothelialize stent struts within 48 h (n = 4). The QuickSeeded stent struts produced significantly more nitric oxide in ex vivo flow circuits after 24 h, as compared to static conditions (n = 5). In conclusion, ECs QuickSeeded onto commercial nitinol stents within minutes of implantation spread to form a functional layer in vitro and in vi...Continue Reading

References

Sep 25, 2003·Circulation·Andrew FarbRenu Virmani
Dec 19, 2003·Journal of Vascular Surgery·Todd R VogelAlan M Graham
Jan 11, 2007·Hypertension·Evangeline D MotleySatoru Eguchi
Jun 22, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Subbu Venkatraman, Freddy Boey
Sep 19, 2007·Journal of Biomedical Materials Research. Part a·Wei ZhuGuo-Yuan Yang
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Apr 19, 2012·Circulation. Arrhythmia and Electrophysiology·Avish NagpalMuhammad R Sohail
Apr 5, 2013·Journal of Biomedical Materials Research. Part a·Alexandra E JantzenGeorge A Truskey
Apr 23, 2013·Annals of Biomedical Engineering·Sa Do KangHardean E Achneck

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Citations

Oct 1, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Rachael Simon-WalkerKetul C Popat
Apr 4, 2017·Neurosurgical Focus·Nam K YoonMin S Park
May 5, 2017·Cardiovascular and Interventional Radiology·Marja Ter MeerLambertus P van den Heuvel
Aug 18, 2021·Stem Cell Reviews and Reports·Jitsuro TsukadaF Vogt

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