Poising of Escherichia coli RNA polymerase and its release from the sigma 38 C-terminal tail for osmY transcription.

Journal of Molecular Biology
Adam Z RosenthalJay D Gralla

Abstract

Bacteria must adapt their transcription to overcome the osmotic stress associated with the gastrointestinal tract of their host. This requires the sigma 38 (rpoS) form of RNA polymerase. Here, chromatin immunoprecipitation experiments show that activation is associated with a poise-and-release mechanism in vivo. A C-terminal tail unique among sigma factors is shown to be required for in vivo recruitment of RNA polymerase to the promoter region prior to osmotic shock. C-terminal domain tail-dependent transcription in vivo can be mimicked by using the intracellular signaling molecule potassium glutamate in vitro. Following signaling, the barrier to elongation into the gene body is overcome and RNA polymerase is released to produce osmY mRNA.

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Jan 20, 2006·Molecular Microbiology·Adam Z RosenthalJay D Gralla
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Citations

Jul 20, 2010·Biochemistry. Biokhimii︠a︡·Yu A KhodakV L Drutsa
Mar 12, 2008·Molecular Microbiology·Adam Z RosenthalJay D Gralla
Mar 1, 2012·Food Microbiology·Sinisa VidovicDarren R Korber
Jan 25, 2017·Journal of Bacteriology·Garrett T WongDaniel M Stoebel
Oct 7, 2010·Molecular Microbiology·Christine ZieglerReinhard Krämer
Dec 1, 2011·EcoSal Plus·Regine Hengge
Aug 1, 2009·EcoSal Plus·Karlheinz AltendorfJanet M Wood
Dec 5, 2008·Biochemistry·Jay D Gralla, Yi-Xin Huo
Jan 20, 2009·Molecular Cell·Rachel A MooneyRobert Landick

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