Pokeweed antiviral protein: its cytotoxicity mechanism and applications in plant disease resistance

Toxins
Rong Di, Nilgun E Tumer

Abstract

Pokeweed antiviral protein (PAP) is a 29 kDa type I ribosome inactivating protein (RIP) found in pokeweed plants. Pokeweed produces different forms of PAP. This review focuses on the spring form of PAP isolated from Phytolacca americana leaves. PAP exerts its cytotoxicity by removing a specific adenine from the α-sarcin/ricin loop of the large ribosomal RNA. Besides depurination of the rRNA, PAP has additional activities that contribute to its cytotoxicity. The mechanism of PAP cytotoxicity is summarized based on evidence from the analysis of transgenic plants and the yeast model system. PAP was initially found to be anti-viral when it was co-inoculated with plant viruses onto plants. Transgenic plants expressing PAP and non-toxic PAP mutants have displayed broad-spectrum resistance to both viral and fungal infection. The mechanism of PAP-induced disease resistance in transgenic plants is summarized.

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Citations

Mar 15, 2016·Biochimica Et Biophysica Acta·Rosario IglesiasJosé M Ferreras
May 15, 2015·Applied Microbiology and Biotechnology·Juan YanZu-Jian Wu
Mar 22, 2018·The Biochemical Journal·David Harrich, Hongping Jin
May 1, 2019·Toxins·Przemysław GrelaMarek Tchórzewski
May 6, 2020·Wiley Interdisciplinary Reviews. RNA·Ricardo A Urquidi CamachoAlbrecht G von Arnim
Jan 28, 2021·Toxins·Lucía CitoresJosé M Ferreras
Jun 1, 2021·Frontiers in Molecular Biosciences·Mikiei Tanaka, P Boon Chock

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Datasets Mentioned

BETA
X86085
and
X98079
AY603353

Methods Mentioned

BETA
electron microscopy
PCR
transgenic
RIPs
surface plasmon resonance
RIP

Software Mentioned

BLAST

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