Polar localization of the ATPase ClpV-5 occurs independent of type VI secretion system apparatus proteins in Burkholderia thailandensis

BMC Research Notes
Jan LenningsSandra Schwarz

Abstract

ClpV, the ATPase of the type VI secretion system (T6SS) recycles cytoplasmic T6SS proteins following effector translocation. Fluorescent protein fusions to ClpV showed that it localizes to discrete and dynamic foci. ClpV-1-sfGFP of the bacterial cell targeting T6SS-1 of Burkholderia thailandensis exhibits a virtually random localization, whereas ClpV-5-sfGFP of the T6SS-5 targeting host cells is located at one or both poles. The mechanisms underlying the differential localization pattern are not known. Previous analysis of T6SSs, which target bacterial cells revealed that ClpV foci formation is dependent on components of the T6SS. Here, we investigated if the T6SS-5 apparatus confers polar localization of ClpV-5. ClpV-5-sfGFP foci formation and localization was examined in a B. thailandensis mutant harboring a deletion of the entire T6SS-5 gene cluster. We found that ClpV-5-sfGFP localization to discrete foci was not abolished in the absence of the T6SS-5 apparatus. Furthermore, the number of ClpV-5-sfGFP foci displaying a polar localization was not significantly different from that of ClpV-5-sfGFP expressed in the wild type genetic background. These findings suggest the presence of a T6SS-independent localization mechanism for...Continue Reading

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Citations

Aug 6, 2019·International Journal of Medical Microbiology : IJMM·Jan LenningsSandra Schwarz
Nov 11, 2021·Applied Microbiology and Biotechnology·Viola Camilla ScoffoneSilvia Buroni

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Methods Mentioned

BETA
PCR

Software Mentioned

BaseClear

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