Polarisation, key to good localisation

Biochimica Et Biophysica Acta
Moniek van BeestPeter M T Deen

Abstract

Polarisation of cells is crucial for vectorial transport of ions and solutes. In literature, however, proteins specifically targeted to the apical or basolateral membrane are often studied in non-polarised cells. To investigate whether these data can be extrapolated to expression in polarised cells, we studied several membrane-specific proteins. In polarised MDCK cells, the Aquaporin-2 water channel resides in intracellular vesicles and apical membrane, while the vasopressin-type 2 receptor, anion-exchanger 1 (AE1) protein and E-Cadherin mainly localise to the basolateral membrane. In non-polarised MDCK cells, however, Aquaporin-2 localises, besides plasma membrane, mainly in the Golgi complex, while the others show a dispersed staining throughout the cell. Moreover, while AQP2 mutants in dominant nephrogenic diabetes insipidus are missorted to different organelles in polarised cells, they all predominantly localise to the Golgi complex in non-polarised MDCK cells. Additionally, the maturation of V2R, and likely its missorting, is affected in transiently-transfected compared to stably-transfected cells. In conclusion, we show that the use of stably-transfected polarised cells is crucial in interpreting the processing and the lo...Continue Reading

References

Feb 18, 1981·Biochimica Et Biophysica Acta·J C RichardsonN L Simmons
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·T KatsuraD Brown
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S NielsenH W Harris
Feb 1, 1997·Journal of the American Society of Nephrology : JASN·S M MuldersP M Deen
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·F E KaretR P Lifton
Jan 29, 1999·Physiological Reviews·C YeamanW J Nelson
Sep 21, 2000·American Journal of Physiology. Renal Physiology·E J Kamsteeg, P M Deen
Nov 13, 2001·American Journal of Physiology. Renal Physiology·G TammaG Valenti
Jan 15, 2002·American Journal of Physiology. Renal Physiology·Peter M T DeenMichael J Caplan
Jan 17, 2002·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·N V Knoers, P M Deen
Feb 5, 2002·Annual Review of Physiology·Seth L Alper
Jul 26, 2002·Journal of the American Society of Nephrology : JASN·Fiona E Karet
Aug 27, 2002·The Journal of Biological Chemistry·Bas W M van BalkomPeter M T Deen
Jan 23, 2003·Nature Genetics·Mark A J DevonaldFiona E Karet
Apr 5, 2003·American Journal of Physiology. Renal Physiology·Dennis Brown
Dec 10, 2003·The Journal of Cell Biology·Erik-Jan KamsteegPeter M T Deen
Sep 1, 2004·Biochemical and Biophysical Research Communications·Yumi NodaSei Sasaki
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Enrique Rodriguez-BoulanAnne Müsch
Apr 13, 2005·Biochemical and Biophysical Research Communications·Yumi NodaSei Sasaki
May 21, 2005·Molecular & Cellular Proteomics : MCP·Maria BarileMark A Knepper
Aug 27, 2005·Journal of the American Society of Nephrology : JASN·Fabrizio de MattiaPeter M T Deen
Nov 4, 2005·Molecular Biology of the Cell·J H RobbenP M T Deen

❮ Previous
Next ❯

Citations

Dec 23, 2011·Cellular and Molecular Life Sciences : CMLS·G TammaG Valenti
Mar 20, 2013·Molecular Aspects of Medicine·Anwar A Khan, John G Quigley
Jan 18, 2011·Biochimica Et Biophysica Acta·Anwar A Khan, John G Quigley
Nov 19, 2015·Journal of the American Society of Nephrology : JASN·Mohammad AlsadyTheun de Groot
Sep 12, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fabian ItelVolker Endeward
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Emmanuelle Cordat
Jan 9, 2020·Molecular Cancer Research : MCR·Christina StanglW P Kloosterman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.