Polarity profiles in oriented and dispersed phosphatidylcholine bilayers are different: an electron spin resonance study

Biophysical Journal
M Ge, Jack H Freed

Abstract

A novel method was utilized to accurately measure the z- component of the nuclear hyperfine interaction tensor, Azz, of a chain-labeled lipid, 16PC, and a headgroup-labeled lipid, dipalmitoylphosphatidyl-tempocholine (DPPTC), in macroscopically oriented dipalmitoylphosphatidylcholine (DPPC) and dimyristoylphosphatidylcholine (DMPC) membranes, which were compared with the Azz values of the two labels in dispersions of the same lipids in the gel phase. We found that the Azz values of 16PC (DPPTC) in the oriented DPPC and DMPC bilayers are approximately 1 Gauss smaller (greater) than in the corresponding dispersions. These results indicate that the headgroup region is more polar in macroscopically oriented bilayers than in dispersions, whereas in the chain region, the order in polarity is reversed. This is consistent with previous results on partial molar volumes in the liquid-crystal phase. Differences in the morphology of the macroscopically oriented and dispersed bilayers, which might be responsible, are discussed. Nonlinear least-squares fits of the electron spin resonance spectra of DPPTC in DPPC show that there is a substantial orienting potential in the headgroup region of dispersions that is lipid phase dependent. However,...Continue Reading

References

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Citations

Feb 11, 2000·Chemistry and Physics of Lipids·M M PincelliA M Gennaro
Jan 4, 2015·Chemistry and Physics of Lipids·Rafael P BarrosoAntonio J Costa-Filho
May 16, 2012·Chemistry and Physics of Lipids·Andrew K Smith, Jack H Freed
Apr 30, 2003·Biophysical Journal·Antonio J Costa-FilhoJack H Freed
Jul 21, 2004·Journal of Biochemical and Biophysical Methods·Jennifer M CarrozzinoMorteza G Khaledi
Apr 2, 2003·Biophysical Journal·Antonio J Costa-FilhoJack H Freed
Nov 29, 2016·Scientific Reports·Luis G M BassoAntonio J Costa-Filho
Dec 18, 2008·Physical Chemistry Chemical Physics : PCCP·Giacomo Martini, Laura Ciani
Jan 14, 2011·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Kouichi Nakagawa
May 17, 2017·Biophysics Reviews·Luis G Mansor BassoAntonio J Costa-Filho
Mar 25, 2017·Biochimica Et Biophysica Acta. Biomembranes·Ernanni D VieiraAntonio J Costa-Filho

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