Polarity, spatial organisation of cytoskeleton, and nuclear division in morphologically altered cells of Schizosaccharomyces pombe

Canadian Journal of Microbiology
M Sipiczki, A Grallert

Abstract

To gain more information about the determination of cell polarity and its relationship to the organisation of cytoskeleton, we have examined the mycelial mutant sep1-1 and the multinucleate multipolar syncytia of the triple mutant sep1-1 spl1-1 cdc4-8 by indirect immunofluorescence techniques. We have found that polarity is predetermined by the shape of the cell. During transition from mitosis to interphase the microtubules of the arising cytoplasmic cytoskeleton gradually form a basket-like pattern that reflects the curvatures of the cell envelope. The presumable growing poles, where actin accumulates, usually correlates with sites where the cell tapers and the microtubules converge. However, no growth can be launched at these sites if the cell surface has not been properly processed. Mitosis and meiosis are not affected significantly by changes in cell morphology and polarity, but larger cells are less effective during sporulation. The azygotic asci produced by multinucleate syncytia frequently contain over 20 ascospores.

References

Jul 23, 1976·Molecular & General Genetics : MGG·P NurseK Nasmyth
Jun 1, 1990·Journal of General Microbiology·K YokoyamaM Miyaji
Jul 1, 1989·Current Genetics·S A MacNeill, P Nurse
Oct 1, 1988·The Journal of Cell Biology·C W JacobsJ R Pringle
Jan 1, 1986·Journal of Cell Science. Supplement·J MarksJ S Hyams

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