Polarization-induced local pore-wall functionalization for biosensing: from micropore to nanopore

Analytical Chemistry
Jie LiuThierry Livache

Abstract

The use of biological-probe-modified solid-state pores in biosensing is currently hindered by difficulties in pore-wall functionalization. The surface to be functionalized is small and difficult to target and is usually chemically similar to the bulk membrane. Herein, we demonstrate the contactless electrofunctionalization (CLEF) approach and its mechanism. This technique enables the one-step local functionalization of the single pore wall fabricated in a silica-covered silicon membrane. CLEF is induced by polarization of the pore membrane in an electric field and requires a sandwich-like composition and a conducting or semiconducting core for the pore membrane. The defects in the silica layer of the micropore wall enable the creation of an electric pathway through the silica layer, which allows electrochemical reactions to take place locally on the pore wall. The pore diameter is not a limiting factor for local wall modification using CLEF. Nanopores with a diameter of 200 nm fabricated in a silicon membrane and covered with native silica layer have been successfully functionalized with this method, and localized pore-wall modification was obtained. Furthermore, through proof-of-concept experiments using ODN-modified nanopores...Continue Reading

References

Aug 1, 1991·IEEE Transactions on Bio-medical Engineering·E OguraT Moriizumi
Sep 15, 2001·Nature·H Bayley, P S Cremer
Sep 2, 2004·Journal of the American Chemical Society·Zuzanna SiwyCharles R Martin
Dec 22, 2004·Accounts of Chemical Research·Takashi ItoRichard M Crooks
Apr 7, 2005·Journal of the American Chemical Society·Zuzanna SiwyCharles R Martin
Sep 15, 2005·Nano Letters·Daniel FologeaJiali Li
Nov 30, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Christophe DanelonHorst Vogel
Jan 16, 2007·Biosensors & Bioelectronics·Young-Rok KimChristopher Ko
May 17, 2007·Nano Letters·Meni Wanunu, Amit Meller
Sep 21, 2007·Molecular BioSystems·Lindsay T SextonCharles R Martin
Dec 12, 2007·Nano Letters·Grigori SigalovAleksei Aksimentiev
Jun 11, 2008·Journal of the American Chemical Society·Fan XiaLei Jiang
Jul 26, 2008·Nature Nanotechnology·Cees Dekker
Jul 26, 2008·Nature Nanotechnology·Samir M IqbalRashid Bashir
Jun 11, 2009·Journal of the American Chemical Society·Ivan VlassioukZuzanna S Siwy
Jul 10, 2009·Small·Aurélie BouchetVincent Haguet
Jul 23, 2009·Chemical Society Reviews·Stefan Howorka, Zuzanna Siwy
Sep 28, 2010·Physical Review Letters·Binquan LuanGlenn Martyna
Feb 22, 2011·Nature Nanotechnology·Erik C YuskoMichael Mayer
Feb 23, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Anmiv S PrabhuMin Jun Kim
May 4, 2011·Journal of the American Chemical Society·Cuiping HanLei Jiang
May 17, 2011·IEEE Transactions on Nanotechnology·Winston TimpGregory Timp

❮ Previous
Next ❯

Citations

May 17, 2013·Nanoscale Research Letters·Lei LiuYunfei Chen
Oct 17, 2019·Biosensors·Aurélie Bouchet-SpinelliPascal Mailley
Dec 10, 2016·Scientific Reports·Timothée MenaisArnaud Buhot
Aug 17, 2020·Sensors·Lucile ReynaudArnaud Buhot
Mar 17, 2021·Analytical Chemistry·Mathias StanzelAnnette Andrieu-Brunsen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.