Polarization of hydroxyapatite: influence on osteoblast cell proliferation

Acta Biomaterialia
D KumarS H Cartmell

Abstract

Hydroxyapatite (HA) has been used clinically to treat bone defects. However, modifications of the surface properties of HA could improve and control bone matrix deposition and localized host tissue integration. The aim of this study was to investigate the effect of developing a surface charge on HA discs with respect to osteoblast activity in vitro. HA discs (12 mm x 2 mm) were sintered in either air or water vapour. The HA discs were then electrically polarized (positive and negative surfaces) or non-polarized (controls) and seeded with MC3T3-E1 cells. Polarized HA sintered in water vapour was shown to retain six times more charge than polarized HA sintered in air. Picogreen analysis demonstrated that at 4h cell number was significantly higher on the negatively and positively charged HA surface (water sintered) in comparison to the non-charged water and air-sintered HA controls. At 7 days there was a significant increase in cell number on the negatively charged HA (air sintered) sample in comparison to the negatively charged water vapour sintered HA sample and the non-charged water vapour sintered control sample. Also at 7 days, the picogreen data showed a significant increase in cell number on the positively charged water-tre...Continue Reading

Citations

Oct 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Solaiman TarafderSusmita Bose
Apr 5, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Ashutosh Kumar Dubey, Ken-ichi Kakimoto
May 8, 2013·Journal of Biomedical Materials Research. Part a·S H CartmellI G Turner
Feb 10, 2012·Journal of Biomedical Materials Research. Part a·Asha RupaniSarah Cartmell
Aug 4, 2011·Journal of Biomedical Materials Research. Part a·Akiko NagaiKimihiro Yamashita
Aug 1, 2012·Journal of Biomedical Materials Research. Part a·M VilaM Vallet-Regi
Jan 26, 2012·Journal of Biomedical Materials Research. Part a·Miho NakamuraKimihiro Yamashita
May 1, 2013·Journal of Biomedical Materials Research. Part a·Leah GarberDaniel Hayes
Oct 28, 2014·Journal of Biomedical Materials Research. Part a·Wing-Hin LeeRamin Rohanizadeh
Oct 25, 2016·Materials Science & Engineering. C, Materials for Biological Applications·M Buchi SureshRoy Johnson
Feb 1, 2020·Science and Technology of Advanced Materials·Fu-Cheng KaoZong-Hong Lin
Sep 14, 2012·Biomedical Materials·Gisela M Luz, João F Mano
Apr 3, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Kate FoxNhiem Tran
Nov 26, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Angaraj SinghAshutosh Kumar Dubey
Feb 19, 2021·Journal of Biomaterials Applications·Angaraj Singh, Ashutosh Kumar Dubey
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Xinrui LiuRen-Jei Chung
Apr 6, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Rashmi GhoshMatthew Z Yates

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.