Polarization of the endomembrane system is an early event in fucoid zygote development

BMC Plant Biology
Rhett HadleyD L Kropf

Abstract

Fucoid zygotes are excellent experimental organisms for investigating mechanisms that establish cell polarity and determine the site of tip growth. A common feature of polarity establishment is targeting endocytosis and exocytosis (secretion) to localized cortical domains. We have investigated the spatiotemporal development of endomembrane asymmetry in photopolarizing zygotes, and examined the underlying cellular physiology. The vital dye FM4-64 was used to visualize endomembranes. The endomembrane system preferentially accumulated at the rhizoid (growth) pole within 4 h of fertilization. The polarized endomembrane array was initially labile and reoriented when the developmental axis changed direction in response to changing light cues. Pharmacological studies indicated that vesicle trafficking, actin and microtubules were needed to maintain endomembrane polarity. In addition, endocytosis required a functional cortical actin cytoskeleton. Endomembrane polarization is an early event in polarity establishment, beginning very soon after photolocalization of cortical actin to the presumptive rhizoid site. Targeting of endocytosis and secretion to the rhizoid cortex contributes to membrane asymmetry. We suggest that microtubule-acti...Continue Reading

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Citations

Mar 12, 2011·Annual Review of Plant Biology·Carolyn G RasmussenLaurie G Smith
Aug 26, 2009·Plant Signaling & Behavior·Rachel A Muzzy, Whitney E Hable
Dec 30, 2014·Frontiers in Plant Science·Whitney E Hable
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Aug 31, 2010·Molecular Reproduction and Development·Whitney E Hable, Peter E Hart
Nov 10, 2006·Trends in Plant Science·Jozef SamajDiedrik Menzel

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Methods Mentioned

BETA
confocal microscopy
GTPases

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