Polarization-sensitive optical coherence tomography imaging of the anterior mouse eye

Journal of Biomedical Optics
Bernhard BaumannChristoph K Hitzenberger

Abstract

Polarization-sensitive optical coherence tomography (PS-OCT) enables noninvasive, high-resolution imaging of tissue polarization properties. In the anterior segments of human eyes, PS-OCT allows the visualization of birefringent and depolarizing structures. We present the use of PS-OCT for imaging the murine anterior eye. Using a spectral domain PS-OCT setup operating in the 840-nm regime, we performed in vivo volumetric imaging in anesthetized C57BL/6 mice. The polarization properties of murine anterior eye structures largely replicated those known from human PS-OCT imagery, suggesting that the mouse eye may also serve as a model system under polarization contrast. However, dissimilarities were found in the depolarizing structure of the iris which, as we confirmed in postmortem histological sections, were caused by anatomical differences between both species. In addition to the imaging of tissues in the anterior chamber and the iridocorneal angle, we demonstrate longitudinal PS-OCT imaging of the murine anterior segment during mydriasis as well as birefringence imaging of corneal pathology in an aged mouse.

References

Nov 26, 1998·The British Journal of Ophthalmology·T R KramerH E Grossniklaus
Jan 13, 2004·Journal of Biomedical Optics·Luigi Rovati, Franco Docchio
Aug 2, 2006·Optics Letters·R A LeitgebT Lasser
Oct 27, 2006·Investigative Ophthalmology & Visual Science·Mika Asai-CoakwellOrdan J Lehmann
Jul 27, 2007·Investigative Ophthalmology & Visual Science·Erich GötzingerChristoph K Hitzenberger
Nov 17, 2007·Optics Letters·Renu TripathiJohannes F de Boer
Jun 3, 2008·Investigative Ophthalmology & Visual Science·Masahiro MiuraYoshiaki Yasuno
Oct 15, 2008·Optics Express·Erich GötzingerChristoph K Hitzenberger
Dec 9, 2008·Investigative Ophthalmology & Visual Science·Boris PovazayWolfgang Drexler
Mar 5, 2009·Optics Express·Erich GötzingerChristoph K Hitzenberger
Mar 7, 2009·Investigative Ophthalmology & Visual Science·Johanna Tukler HenrikssonJan P G Bergmanson
Apr 22, 2009·Brain Research Bulletin·Thomas V Johnson, Stanislav I Tomarev
Nov 29, 2004·Optics Express·Michael PircherChristoph Hitzenberger
Dec 12, 2005·Optics Express·Erich GötzingerChristoph K Hitzenberger
Feb 5, 2007·Optics Express·Bernhard BaumannChristoph K Hitzenberger
Oct 3, 2009·Investigative Ophthalmology & Visual Science·Christian AhlersUrsula Schmidt-Erfurth
Mar 5, 2011·Investigative Ophthalmology & Visual Science·Tsung-Han ChouVittorio Porciatti
Jul 7, 2011·Progress in Retinal and Eye Research·Michael PircherUrsula Schmidt-Erfurth
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 19, 2012·Biomedical Optics Express·Bernhard BaumannChristoph K Hitzenberger
Jan 26, 2013·Investigative Ophthalmology & Visual Science·Shinichi FukudaYoshiaki Yasuno
Feb 16, 2013·Biomedical Optics Express·Jonathan J LiuJames G Fujimoto
Feb 15, 2014·Investigative Ophthalmology & Visual Science·Jan LammerUrsula Schmidt-Erfurth
Oct 30, 2014·Investigative Ophthalmology & Visual Science·Bernhard BaumannChristoph K Hitzenberger
Aug 26, 2015·Investigative Ophthalmology & Visual Science·Demelza R KoehnMichael G Anderson

❮ Previous
Next ❯

Citations

Dec 5, 2019·Biomedical Optics Express·Bernhard BaumannChristoph K Hitzenberger
Jul 3, 2021·NPJ Digital Medicine·Yi SunStephen A Boppart

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.