Polarized activities of AMPK and BRSK in primary hippocampal neurons

Molecular Biology of the Cell
Vedangi SampleJin Zhang

Abstract

5'-Adenosine monophosphate-activated protein kinase (AMPK) is a master metabolic regulator that has been shown to inhibit the establishment of neuronal polarity/axogenesis under energy stress conditions, whereas brain-specific kinase (BRSK) promotes the establishment of axon-dendrite polarity and synaptic development. However, little information exists regarding the localized activity and regulation of these kinases in developing neurons. In this study, using a fluorescence resonance energy transfer (FRET)-based activity reporter that responds to both AMPK and BRSK, we found that BRSK activity is elevated in the distal region of axons in polarized hippocampal neurons before any stimulation and does not respond to either Ca(2+) or 2-deoxyglucose (2-DG) stimulation. In contrast, AMPK activity is stimulated by either Ca(2+) or 2-DG in the soma, dendrites, and axons of hippocampal neurons, with maximal stimulated activity observed in the distal region of the axon. Our study shows that the activities of both AMPK and BRSK are polarized in developing hippocampal neurons, with high levels in the distal region of extended axons.

References

Dec 18, 1998·The Journal of Biological Chemistry·B E CruteL A Witters
Nov 14, 2002·Molecular Biology of the Cell·Jacek BiernatEva-Maria Mandelkow
Dec 10, 2002·Science·G ManningS Sudarsanam
Jan 4, 2003·The Journal of Clinical Investigation·Benoit ViolletSophie Vaulont
Feb 12, 2005·Science·Masashi KishiJoshua R Sanes
Feb 19, 2005·Science·D D SarbassovDavid M Sabatini
Dec 17, 2005·Trends in Biochemical Sciences·Lee A WittersAnthony R Means
Jun 8, 2006·Annual Review of Biochemistry·Dario R AlessiJose R Bayascas
Jul 28, 2006·Biochemical and Biophysical Research Communications·Zekun GuoLong Yu
Aug 10, 2006·Biochemical and Biophysical Research Communications·Michael D Allen, Jin Zhang
Oct 16, 2007·Journal of Neuroscience Methods·Amy M KlemanLeslie E Landree
Mar 8, 2008·Biochemistry·Tomohito FujimotoHiroshi Tokumitsu
Mar 15, 2008·The Journal of Biological Chemistry·Nicola J BrightClaire Thornton
Sep 12, 2008·Molecular Biology of the Cell·Chinten J LimMark H Ginsberg
Sep 17, 2008·Genes & Development·Yong-Jin ChoiXi He
May 7, 2009·Journal of Neurochemistry·Gabriele V RonnettSusan Aja
Sep 15, 2010·Molecular BioSystems·Charlene DepryJin Zhang
Mar 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tyisha WilliamsFranck Polleux
Apr 5, 2011·Cell Metabolism·Peiling TsouLewis C Cantley
Jul 26, 2011·Trends in Biochemical Sciences·D Grahame HardieSteven J Gamblin
Sep 6, 2011·Nature Cell Biology·Maria M Mihaylova, Reuben J Shaw
Mar 23, 2012·Nature Reviews. Molecular Cell Biology·D Grahame HardieSimon A Hawley
Jun 19, 2012·The Biochemical Journal·David CarlingMatthew J Sanders
Jul 11, 2012·The Journal of Biological Chemistry·Luigi Racioppi, Anthony R Means
Jan 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Brendan N LilleyJoshua R Sanes

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Citations

Dec 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sarah A HeadJun O Liu
Nov 10, 2015·Chemistry & Biology·Charlene DepryJin Zhang
Jun 1, 2016·Molecular Membrane Biology·Audrey Montersino, Gareth M Thomas
Jan 24, 2017·Neuron·Ghazaleh AshrafiTimothy A Ryan
Sep 12, 2015·Oncotarget·Takafumi MiyamotoTakanari Inoue
Oct 6, 2020·Biomedical Optics Express·Shashi Bhushan SrivastavaSedat Nizamoglu
Feb 15, 2021·Human Reproduction·Anne-Lotte L F van der KooiEline van Dulmen-den Broeder
Jul 15, 2020·Trends in Biochemical Sciences·Xin ZhouJin Zhang

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Methods Mentioned

BETA
fluorescence resonance
FRET
PCR
Fluorescence
transfection
fluorescence microscopy

Software Mentioned

ImageJ
METAFLUOR

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