Polarized ATP distribution in urothelial mucosal and serosal space is differentially regulated by stretch and ectonucleotidases

American Journal of Physiology. Renal Physiology
Weiqun Yu

Abstract

Purinergic signaling is a major pathway in regulating bladder function, and mechanical force stimulates urothelial ATP release, which plays an important role in bladder mechanotransduction. Although urothelial ATP release was first reported almost 20 years ago, the way in which release is regulated by mechanical force, and the presence of ATP-converting enzymes in regulating the availability of released ATP is still not well understood. Using a set of custom-designed Ussing chambers with the ability to manipulate mechanical forces applied on the urothelial tissue, we have demonstrated that it is stretch and not hydrostatic pressure that induces urothelial ATP release. The experiments reveal that urothelial ATP release is tightly controlled by stretch speed, magnitude, and direction. We have further shown that stretch-induced urothelial ATP release is insensitive to temperature (4°C). Interestingly, stretch-induced ATP release shows polarized distribution, with the ATP concentration in mucosal chamber (nanomolar level) about 10 times higher than the ATP concentration in serosal chamber (subnanomolar level). Furthermore, we have consistently observed differential ATP lifetime kinetics in the mucosal and serosal chambers, which is...Continue Reading

References

Jan 1, 1989·Methods in Cell Biology·E Kuismanen, J Saraste
Jun 3, 2000·American Journal of Physiology. Renal Physiology·S A Lewis
Jul 12, 2002·American Journal of Physiology. Renal Physiology·John LavelleMark L Zeidel
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·Edward WangGerard Apodaca
Apr 17, 2004·Traffic·Gerard Apodaca
Apr 30, 2005·The Journal of Physiology·Haibing Teng, Robert S Wilkinson
Aug 20, 2005·The Journal of Clinical Investigation·Edward C Y WangGerard Apodaca
Mar 31, 2006·American Journal of Physiology. Cell Physiology·Weiqun YuGerard Apodaca
Apr 7, 2006·American Journal of Physiology. Renal Physiology·Simon A Lewis, Jamie R Lewis
Aug 2, 2007·Kidney International·G ApodacaL A Birder
Jan 23, 2009·American Journal of Physiology. Renal Physiology·F Aura KullmannW C de Groat
Oct 15, 2010·American Journal of Physiology. Renal Physiology·Weiqun YuMark L Zeidel
Dec 17, 2010·American Journal of Physiology. Renal Physiology·Kazumasa Matsumoto-MiyaiMasahito Kawatani
May 25, 2012·Trends in Neurosciences·Bernd Nilius, Eric Honoré
Jul 14, 2012·Biomedical Research·Kazumasa Matsumoto-MiyaiMasahito Kawatani
Jul 17, 2012·Journal of Cell Science·Sergei Sukharev, Frederick Sachs
Feb 12, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keizo KanasakiWarren G Hill
Oct 15, 2013·Cell·Patrick Delmas, Bertrand Coste
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Apr 11, 2014·American Journal of Physiology. Renal Physiology·Weiqun YuMark L Zeidel
Apr 25, 2014·The Journal of Biological Chemistry·Tatsuya MiyamotoMakoto Tominaga
Jan 30, 2015·The Journal of Physiology·Jonathan M BeckelWilliam C de Groat

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Citations

Oct 30, 2016·Basic & Clinical Pharmacology & Toxicology·Christopher H Fry, Bahareh Vahabi
Aug 20, 2016·Neurourology and Urodynamics·Kentaro TakezawaShoichi Shimada
Mar 22, 2018·American Journal of Physiology. Renal Physiology·Nicolas MontalbettiMarcelo D Carattino
Jul 29, 2016·American Journal of Physiology. Renal Physiology·Leonie DurninVioleta N Mutafova-Yambolieva
Jul 15, 2016·Scientific Reports·Hiroshi NakagomiSchuichi Koizumi
Jun 14, 2017·BJU International·Darryl G KitneyChristopher H Fry
Mar 21, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiang XieWeiqun Yu
Jun 15, 2021·Frontiers in Cellular and Infection Microbiology·Benjamin O MurrayJennifer L Rohn
Jul 31, 2021·Autonomic Neuroscience : Basic & Clinical·Christopher H Fry, Karen D McCloskey

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