Polarized endocytosis and transcytosis in the hypothalamic tanycytes of the rat

Cell and Tissue Research
Bruno PeruzzoEsteban M Rodríguez

Abstract

Four types of tanycytes can be distinguished in the rat hypothalamus: alpha(1) and alpha(2) tanycytes establish an anatomical link between the ventricular cerebrospinal fluid (CSF) and the arcuate nucleus, whereas beta(1) and beta2 tanycytes establish a link between CSF and portal blood. Endocytosis and transcytosis in these cells have been investigated by (1) immunocytochemistry with antibodies against molecular markers of the endocytotic and transcytotic pathways; (2) the administration of wheat germ agglutinin (WGA) into the ventricular or subarachnoidal CSF and following its internalisation by and its routing through tanycytes. The four populations of tanycytes show marked differences concerning the expression and subcellular location of proteins involved in endocytosis and transcytosis, such as clathrin, caveolin-1, Rab4 and ARF6. Thus, beta1,2 tanycytes express caveolin-1 at the ventricular cell pole and at their terminals contacting the portal capillaries, whereas alpha1,2 tanycytes do not, suggesting that caveolae-dependant endocytosis does not occur in the latter and that, in beta1,2 tanycytes, it may occur at both cell poles. In beta1,2 tanycytes, clathrin is only expressed at the ventricular cell pole indicating that...Continue Reading

Citations

Mar 4, 2008·Cerebrospinal Fluid Research·Olga A de WitDeborah A Sival
Feb 17, 2010·Cerebrospinal Fluid Research·Jan G Veening, Henk P Barendregt
Sep 30, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Brooke N HortonAgnieszka A Ardelt
Sep 24, 2013·Cell and Tissue Research·Bastienne BrauksiepeErwin R Schmidt
Dec 5, 2014·Biologie aujourd'hui·Fanny Langlet
May 25, 2010·Physiology & Behavior·Jan G VeeningHenk P Barendregt
Oct 1, 2008·Biochemical and Biophysical Research Communications·Xiaoling GaoXinguo Jiang
Sep 28, 2015·Frontiers in Neuroendocrinology·Eglantine Balland, Michael A Cowley
May 8, 2013·The Journal of Comparative Neurology·Fanny LangletBenedicte Dehouck
Aug 19, 2014·Journal of Neuroendocrinology·F Langlet
Sep 30, 2014·The Journal of Comparative Neurology·Rajamani SelvamWayne J Kuenzel
Dec 3, 2014·Free Radical Biology & Medicine·Rachel C LazarusGregory P Mueller
Apr 11, 2015·Frontiers in Endocrinology·Pilar Argente-ArizónJulie A Chowen
Dec 25, 2012·Autonomic Neuroscience : Basic & Clinical·Hreday N Sapru
Feb 11, 2014·Cell Metabolism·Eglantine BallandVincent Prévot
Dec 14, 2005·International Review of Cytology·Esteban M RodríguezPedro Amat
May 12, 2011·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Mari Sild, Edward S Ruthazer
Jan 20, 2018·Endocrine Reviews·Vincent PrevotJerome Clasadonte
Jul 20, 2005·Pharmaceutical Research·Conrad E JohansonAndrew Baird
Jan 31, 2019·Journal of Neuroendocrinology·Esteban RodríguezJuan Luis Blázquez
Sep 15, 2018·Endocrine Reviews·José Cipolla-Neto, Fernanda Gaspar do Amaral
May 5, 2011·American Journal of Physiology. Endocrinology and Metabolism·Christian RoyKatherine Cianflone
Jul 27, 2005·Journal of Neuropathology and Experimental Neurology·María Dolores Domínguez-PinosEsteban-Martin Rodríguez
Nov 19, 2015·Frontiers in Neuroscience·Timothy Goodman, Mohammad K Hajihosseini
Apr 4, 2019·Frontiers in Neuroscience·Sooyeon YooSeth Blackshaw
Apr 1, 2008·Neurochemical Research·Qing-Ping WangSeiji Shioda
Jul 12, 2019·Frontiers in Endocrinology·Adair Rodríguez-RodríguezJean-Louis Charli
May 2, 2019·Frontiers in Endocrinology·Roberto Javier Elizondo-VegaKarina Oyarce
Apr 18, 2017·Cell and Tissue Research·Pablo Nicolás De FrancescoMario Perello

❮ Previous
Next ❯

Related Concepts

Related Feeds

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.