Polarized Epithelial Cells Secrete Interleukin 6 Apically in the Bovine Endometrium

Biology of Reproduction
Laura L HealyI Martin Sheldon

Abstract

Endometrial epithelial cells are the first line of defense against pathogenic bacteria infecting the uterus. Innate immune responses by these polarized epithelial cells to bacteria and tissue damage are characterized by release of the chemokine (C-X-C motif) ligand 8 (CXCL8) to attract immune cells from the circulation to the site of infection, where they are regulated by the cytokine interleukin (IL) 6. The present study tested the hypothesis that IL6 is predominantly secreted apically from polarized bovine endometrial epithelial cells in response to stimuli associated with bacterial infection and tissue damage. In postpartum animals, concentrations of IL6, but not of CXCL8, were higher in uterine mucus than in peripheral blood. In vitro, polarized endometrial epithelial cells only secreted IL6 apically when treated with bacteria, the pathogen-associated molecule lipopolysaccharide, or the damage-associated molecule IL1alpha, whereas CXCL8 accumulated apically and basolaterally. Furthermore, IL6 accumulated apically irrespective of whether lipopolysaccharide was applied to the apical or basolateral surface of epithelial cells. Secretion of IL6 from epithelial cells was dependent on the trans-Golgi network but was not affected ...Continue Reading

References

Aug 1, 1988·Endocrinologia Japonica·C W WeemsD L Vincent
Apr 1, 1972·Journal of Reproduction and Fertility·L F ArchbaldR Zemjanis
Sep 1, 2000·Theriogenology·C FourichonX Malher
Jun 8, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·I M SheldonH Dobson
Dec 24, 2002·Trends in Immunology·Gilles KaplanskiCatherine Farnarier
Dec 3, 2003·Reproductive Biology and Endocrinology : RB&E·Gregory S Lewis
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Enrique Rodriguez-BoulanAnne Müsch
Jan 3, 2006·The Journal of Infectious Diseases·Matthew R JonesJoseph P Mizgerd
Jul 4, 2007·The Journal of Cell Biology·Anthony P MandersonJennifer L Stow
Oct 24, 2008·Nature Reviews. Molecular Cell Biology·Ira Mellman, W James Nelson
Mar 9, 2010·Theriogenology·Timothy J PotterI Martin Sheldon
Mar 23, 2010·Cell·Osamu Takeuchi, Shizuo Akira
Sep 3, 2010·Cell and Tissue Research·Robert Brownlie, Brenda Allan
Dec 24, 2010·Reproductive Biology and Endocrinology : RB&E·Christoph GablerWolfgang Heuwieser
Jul 12, 2011·Current Biology : CB·Eva Marie Y MorescoBruce Beutler
Feb 14, 2012·American Journal of Reproductive Immunology : AJRI·Alan Maia BorgesIain Martin Sheldon
Mar 1, 2012·Nature Reviews. Immunology·J Magarian Blander, Leif E Sander
Nov 2, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Siân B MacKintoshI Martin Sheldon
Feb 26, 2013·Nature Reviews. Immunology·Elzbieta Kolaczkowska, Paul Kubes
Jun 4, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·I Martin SheldonHilary Dobson
Jul 18, 2014·Journal of Clinical Pathology·Parvin Ataie-KachoieDavid L Morris

❮ Previous
Next ❯

Citations

Mar 5, 2016·Mediators of Inflammation·Natalia Reglero-RealJaime Millán
Mar 10, 2016·Reproductive Biology·Luísa Cunha CarneiroIain Martin Sheldon
Jul 19, 2016·Cell Reports·Niloufar MonhaseryJürgen Scheller
Aug 9, 2016·Journal of Reproductive Immunology·Iain Martin SheldonMatthew Lloyd Turner
Jan 11, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carolina SerranoPorfirio Nava
Feb 3, 2019·BMC Developmental Biology·Lydia K Wooldridge, Alan D Ealy
Aug 18, 2018·Veterinary Research Communications·Mounir AdnaneCliona O'Farrelly
Jul 19, 2017·Trends in Cancer·Yoichiro Tamori, Wu-Min Deng

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
fluorescence-activated cell sorting

Software Mentioned

SPSS

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here