Polarized expression of Ca2+ channels in pancreatic and salivary gland cells. Correlation with initiation and propagation of [Ca2+]i waves.

The Journal of Biological Chemistry
M G LeeS Muallem

Abstract

In polarized epithelial cells [Ca2+]i waves are initiated in discrete regions and propagate through the cytosol. The structural basis for these compartmentalized and coordinated events are not well understood. In the present study we used a combination of [Ca2+]i imaging at high temporal resolution, recording of Ca2+-activated Cl- current, and immunolocalization by confocal microscopy to study the correlation between initiation and propagation of [Ca2+]i waves and localization of Ca2+ release channels in pancreatic acini and submandibular acinar and duct cells. In all cells Ca2+ waves are initiated in the luminal pole and propagate through the cell periphery to the basal pole. All three cell types express the three known inositol 1,4,5-trisphosphate receptors (IP3Rs). Expression of IP3Rs was confined to the area just underneath the luminal and lateral membranes, with no detectable receptors in the basal pole or other regions of the cells. In pancreatic acini and SMG ducts IP3R3 was also found in the nuclear envelope. Expression of ryanodine receptor was detected in submandibular salivary gland cells but not pancreatic acini. Accordingly, cyclic ADP ribose was very effective in mobilizing Ca2+ from internal stores of submandibul...Continue Reading

References

Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jul 28, 1995·The Journal of Biological Chemistry·L Y BourguignonN R Brandt
Jan 1, 1994·Annual Review of Physiology·O H PetersenH Kasai
Jan 28, 1993·Nature·M J Berridge
Oct 4, 1996·The Journal of Biological Chemistry·X XuS Muallem
Aug 1, 1996·Cell Calcium·M J Berridge

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Citations

Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·O H PetersenA V Tepikin
Aug 15, 2000·Journal of Cellular Physiology·K Lange
Jul 27, 2005·Pflügers Archiv : European journal of physiology·Kirill KiselyovShmuel Muallem
Apr 12, 2012·Pflügers Archiv : European journal of physiology·Ole H Petersen
Dec 15, 2010·Neurochemical Research·Dongki YangShmuel Muallem
May 3, 2011·Neurochemical Research·Indu S Ambudkar
Jun 12, 2002·Biochemical and Biophysical Research Communications·Allison F O'NeillBarbara E Ehrlich
Feb 14, 2002·Biochemical Pharmacology·C Camello-AlmarazP J Camello
Oct 24, 2003·Cell Calcium·Jason I E BruceDavid I Yule
Jan 18, 2003·Cellular Signalling·Kirill KiselyovShmuel Muallem
Sep 22, 2000·The Biochemical Journal·T J FitzsimmonsS J Pandol
Nov 19, 2010·The Journal of Biological Chemistry·Anamika M ReedMichael H Nathanson
Oct 31, 2000·The Journal of General Physiology·J S Marchant, I Parker
Feb 1, 2012·The Journal of General Physiology·Janos AlmassyDavid I Yule
Jun 17, 1998·Molecular Biology of the Cell·B S WilsonR J Wojcikiewicz
Feb 28, 2001·The EMBO Journal·J S Marchant, I Parker
Oct 20, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Seung Hyun Yoo
Oct 7, 2006·Current Opinion in Gastroenterology·J A Williams
Feb 16, 2005·Annual Review of Physiology·James E MelvinTed Begenisich
Oct 1, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hyung Seo ParkDavid I Yule
Feb 3, 2012·Physiological Reviews·Min Goo LeeShmuel Muallem
Feb 7, 2001·Biochemical and Biophysical Research Communications·W Masuda, T Noguchi

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