Polarized Raman spectroscopy of double-stranded RNA from bacteriophage phi6: local Raman tensors of base and backbone vibrations

Biophysical Journal
J M BenevidesG J Thomas

Abstract

Raman tensors for localized vibrations of base (A, U, G, and C), ribose and phosphate groups of double-stranded RNA have been determined from polarized Raman measurements on oriented fibers of the genomic RNA of bacteriophage phi6. Polarized Raman intensities for which electric vectors of both the incident and scattered light are polarized either perpendicular (I[bb]) or parallel (I[cc]) to the RNA fiber axis have been obtained by Raman microspectroscopy using 514.5-nm excitation. Similarly, the polarized Raman components, I(bc) and I(cb), for which incident and scattered vectors are mutually perpendicular, have been obtained. Spectra collected from fibers maintained at constant relative humidity in both H2O and D2O environments indicate the effects of hydrogen-isotopic shifts on the Raman polarizations and tensors. Novel findings are the following: 1) the intense Raman band at 813 cm(-1), which is assigned to phosphodiester (OPO) symmetrical stretching and represents the key marker of the A conformation of double-stranded RNA, is characterized by a moderately anisotropic Raman tensor; 2) the prominent RNA band at 1101 cm(-1), which is assigned to phosphodioxy (PO2-) symmetrical stretching, also exhibits a moderately anisotropi...Continue Reading

Citations

May 22, 2010·Analytical and Bioanalytical Chemistry·Florence DrauxGanesh D Sockalingum
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·G J Thomas
Mar 14, 2009·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Masamichi TsuboiGeorge J Thomas
Feb 3, 2016·ACS Nano·Judit Morla-FolchLuca Guerrini
May 21, 2005·Biochimica Et Biophysica Acta·Chongsoo Lee, Colin D Bain
May 5, 2009·Methods : a Companion to Methods in Enzymology·Bo GongPhilip C Bevilacqua
Oct 22, 2015·Physical Chemistry Chemical Physics : PCCP·Rene CostardBenjamin P Fingerhut
Feb 5, 2013·Current Protocols in Protein Science·Daniel NemecekGeorge J Thomas
Oct 8, 2003·The Journal of Biological Chemistry·Jíri LísalRoman Tuma
Jul 5, 2013·Physical Chemistry Chemical Physics : PCCP·Alison J HobroNicholas I Smith
Feb 25, 2009·The Analyst·Florence DrauxGanesh D Sockalingum
Apr 23, 2008·Current Protocols in Protein Science·James M BenevidesGeorge J Thomas
Feb 5, 2021·Scientific Reports·Giuseppe PezzottiOsam Mazda
Jun 26, 1998·Analytical Chemistry·L A LyonM J Natan
Jun 27, 1998·Journal of Molecular Biology·J T JuutiG J Thomas
Aug 28, 2021·Systems Biology in Reproductive Medicine·Sanaz AlaeeTahereh Talaei-Khozani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Christoph KrafftReiner Salzer
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
José J CárcamoMarcelo M Campos-Vallette
Proceedings of the National Academy of Sciences of the United States of America
Kenny KongIoan Notingher
Current Protocols in Nucleic Acid Chemistry
A R Ferré-D'Amaré, J A Doudna
© 2021 Meta ULC. All rights reserved