Polarized release of hepatic microRNAs into bile and serum in response to cellular injury and impaired liver function

Liver International : Official Journal of the International Association for the Study of the Liver
Cornelia J VerhoevenLuc J W van der Laan

Abstract

Extracellular microRNAs (miRNAs) in serum and bile are currently under intense investigation for biomarker purposes in liver disease. However, the directions and pathways by which miRNAs are released from hepatic cells remains largely unknown. Here, we investigated the release of hepatocyte and cholangiocyte-derived miRNAs (HDmiRs and CDmiRs) into blood and bile during various (patho)physiological hepatic conditions. MiRNA release was analysed using longitudinally collected tissue and paired bile and serum samples (n = 124) that were obtained from liver transplant recipients during follow-up. Cell-type specificity of HDmiRs and CDmiRs was confirmed in liver and common bile duct biopsies (P < 0.001). Analysis of paired bile and serum samples showed up to 20-times higher miRNA-levels in bile compared to serum (P < 0.0001). Fractionation of bile showed the majority of miRNAs being present in the unpelletable supernatant, where protein conjunctions protect miRNAs against degradation (P < 0.0001). During episodes of liver injury and histologically proven rejection in liver transplant recipients, relative HDmiR-levels in bile decreased while its levels in serum increased (P ≤ 0.015). Simultaneously, relative CDmiR-levels in bile sign...Continue Reading

References

Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Sep 17, 2004·Nature·Victor Ambros
Dec 2, 2006·Journal of Hepato-biliary-pancreatic Surgery·Carlijn I BuisRobert J Porte
Sep 29, 2007·The American Journal of Gastroenterology·Jean-Marc DumonceauAntoine Hadengue
Jul 31, 2008·Stem Cell Reviews·Peter J Quesenberry, Jason M Aliotta
Dec 17, 2008·Journal of Hepatology·Lei ChenHong-Yang Wang
Jan 23, 2009·Nature·Martin Jinek, Jennifer A Doudna
Feb 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kai WangDavid J Galas
Mar 7, 2009·PloS One·Alex YuanDebora B Farber
Sep 12, 2009·Clinical Chemistry·Omar F LaterzaWarren E Glaab
Oct 14, 2009·Current Opinion in Nephrology and Hypertension·Giovanni CamussiCiro Tetta
Jul 10, 2010·Nucleic Acids Research·Kai WangDavid J Galas
Aug 13, 2010·Kidney International·Giovanni CamussiLuigi Biancone
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jason D ArroyoMuneesh Tewari
Sep 21, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Waqar R R FaridLuc J W van der Laan
Mar 3, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jesús M BanalesNicholas F LaRusso
Nov 30, 2013·The Journal of International Medical Research·Yao RuoquanHuang Feizhou
Feb 6, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ling LiFlorin M Selaru
Mar 22, 2014·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Tim O LankischThomas Thum
Apr 30, 2014·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Cornelia J VerhoevenLuc J W van der Laan
May 7, 2014·Journal of Hepatology·Cornelia J VerhoevenLuc J W van der Laan
Jun 26, 2014·Transplant International : Official Journal of the European Society for Organ Transplantation·Waqar R R FaridLuc J W van der Laan
Jul 17, 2014·Nature Reviews. Molecular Cell Biology·Minju Ha, V Narry Kim
Jul 23, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Henk P RoestLuc J W van der Laan
Jun 20, 2015·Cell·Toshiro Sato, Hans Clevers

❮ Previous
Next ❯

Citations

Apr 8, 2017·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Jasmijn W SeltenLuc J W van der Laan
Apr 5, 2020·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Isabel M A BrüggenwirthPaulo N Martins
Nov 20, 2016·Journal of Veterinary Internal Medicine·K DirksenB Spee
Jun 9, 2020·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Taisa J KohutBrendan J Keating

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Transplant International : Official Journal of the European Society for Organ Transplantation
Waqar R R FaridLuc J W van der Laan
Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
Waqar R R FaridLuc J W van der Laan
© 2021 Meta ULC. All rights reserved