Polarized retinal pigment epithelium generates electrical signals that diminish with age and regulate retinal pathology

Journal of Cellular and Molecular Medicine
Lin CaoNoemi Lois

Abstract

The transepithelial potential difference (TEP) across the retinal pigment epithelial (RPE) is dependent on ionic pumps and tight junction "seals" between epithelial cells. RPE cells release neurotrophic growth factors such as pigment epithelial derived factor (PEDF), which is reduced in age-related macular degeneration (AMD). The mechanisms that control the secretion of PEDF from RPE cells are not well understood. Using the CCL2/CX3CR1 double knockout mouse model (DKO), which demonstrates RPE damage and retinal degeneration, we uncovered an interaction between PEDF and the TEP which is likely to play an important role in retinal ageing and in the pathogenesis of AMD. We found that: (a) the expression of ATP1B1 (the Na+ /K+ -ATPase β1 subunit) was reduced significantly in RPE from aged mice, in patients with CNV (Choroidal Neovascularization) and in DKO mice; (b) the expression of PEDF also was decreased in aged persons and in DKO mice; (c) the TEP across RPE was reduced markedly in RPE cells from DKO mice and (d) an applied electric field (EF) of 50-100 mV/mm, used to mimic the natural TEP, increased the expression and secretion of PEDF in primary RPE cells. In conclusion, the TEP across the RPE depends on the expression of ATP...Continue Reading

References

Jul 1, 1979·The Journal of Experimental Zoology·R B BorgensL F Jaffe
Jun 11, 1992·Experimental Eye Research·M ChiangJ W Vanable
Sep 1, 1991·Experimental Eye Research·J Tombran-TinkL V Johnson
Jul 1, 1988·Trends in Neurosciences·D M Fambrough
Aug 29, 1980·Biochemical and Biophysical Research Communications·A S ReevesA Schwartz
Jul 1, 1984·Clinics in Dermatology·L F Jaffe, J W Vanable
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F R SteeleJ Tombran-Tink
Aug 1, 1995·Protein Expression and Purification·Y Q WuS P Becerra
Feb 1, 1997·Nature Biotechnology·S KoyamaM Aizawa
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M ZhaoC D McCaig
Jul 20, 1999·Journal of Neuropathology and Experimental Neurology·M M BilakR W Kuncl
Jun 26, 2001·Journal of Cellular Physiology·K MoriP A Campochiaro
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·M SimonovicK Volz
Nov 13, 2002·Archives of Ophthalmology·Joshua L DunaiefAnn H Milam
Sep 26, 2003·Investigative Ophthalmology & Visual Science·Hiroyasu TakitaKeisuke Mori
Oct 21, 2003·Nature Medicine·Jayakrishna AmbatiBalamurali K Ambati
Jan 9, 2004·Current Topics in Developmental Biology·Richard Nuccitelli
Feb 25, 2005·Journal of Cell Science·Jin Pu, Min Zhao
Aug 10, 2006·Molecular and Cellular Biochemistry·Toshihiko KojimaIkuo Morita
Aug 31, 2006·Methods in Enzymology·Tanya Barrett, Ron Edgar
Jan 31, 2007·BMC Neuroscience·Iok-Hou PangAbbot F Clark
Jul 27, 2007·Investigative Ophthalmology & Visual Science·Jingsheng TuoChi-Chao Chan
Oct 3, 2007·The Journal of Clinical Investigation·Christophe CombadièreFlorian Sennlaub
Apr 19, 2008·Investigative Ophthalmology & Visual Science·Mei ChenHeping Xu
Apr 19, 2008·Ophthalmic Research·Chi-Chao ChanJingsheng Tuo
Jan 17, 2009·Seminars in Cell & Developmental Biology·Min Zhao
Nov 20, 2009·Journal of Cell Science·Colin D McCaigAnn M Rajnicek
May 12, 2010·Experimental Eye Research·Olga Lucia GamboaNoemi Lois
Nov 20, 2010·Investigative Ophthalmology & Visual Science·Danhong ZhuDavid R Hinton
Jun 11, 2011·Biochimica Et Biophysica Acta·Patricia D Wilson

❮ Previous
Next ❯

Citations

Jun 3, 2021·International Journal of Molecular Sciences·Ivan T RebustiniS Patricia Becerra
Dec 12, 2020·Genomics, Proteomics & Bioinformatics·Grace E LidgerwoodAlice Pébay

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE29801
GSE10965

Methods Mentioned

BETA
dissecting
ELISA
cochlear implants

Software Mentioned

ASET
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.