PMID: 11607619Dec 19, 1995Paper

Pollen selection: a transgenic reconstruction approach

Proceedings of the National Academy of Sciences of the United States of America
A TouraevE Heberle-Bors

Abstract

A transgenic reconstruction experiment has been performed to determine the feasibility of male gametophytic selection to enhance transmission of genes to the next sporophytic generation. For tobacco pollen from a transgenic plant containing a single hygromycin-resistance (hygromycin phosphotransferase, hpt-) gene under control of the dc3 promoter, which is active in both sporophytic and gametophytic tissues, 3 days of in vitro maturation in hygromycin-containing medium was sufficient to result in a 50% reduction of germinating pollen, as expected for meiotic segregation of a single locus insert. Pollination of wild-type plants with the selected pollen yielded 100% transgenic offspring, as determined by the activity of the linked kanamycin-resistance gene--present within the same transferred T-DNA borders--under control of the nos promoter. This is direct proof that selection acting on male gametophytes can be a means to alter the frequency of genes in the progeny.

Citations

Dec 13, 2005·Plant Cell Reports·Danilo D FernandoJulie R Kikkert
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·B Ylstra, S McCormick
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Loverine P. Taylor, Peter K. Hepler
Feb 28, 2004·FEBS Letters·Viktor VoroninCathal Wilson
Feb 21, 2004·Plant Physiology·Sergei A FilichkinHiroyuki Nonogaki

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