Polo kinase interacts with RacGAP50C and is required to localize the cytokinesis initiation complex.

The Journal of Biological Chemistry
Saman EbrahimiStephen L Gregory

Abstract

The assembly and constriction of an actomyosin contractile ring in cytokinesis is dependent on the activation of Rho at the equatorial cortex by a complex, here termed the cytokinesis initiation complex, between a microtubule-associated kinesin-like protein (KLP), a member of the RacGAP family, and the RhoGEF Pebble. Recently, the activity of the mammalian Polo kinase ortholog Plk1 has been implicated in the formation of this complex. We show here that Polo kinase interacts directly with the cytokinesis initiation complex by binding RacGAP50C. We find that a new domain of Polo kinase, termed the intermediate domain, interacts directly with RacGAP50C and that Polo kinase is essential for localization of the KLP-RacGAP centralspindlin complex to the cell equator and spindle midzone. In the absence of Polo kinase, RacGAP50C and Pav-KLP fail to localize normally, instead decorating microtubules along their length. Our results indicate that Polo kinase directly binds the conserved cytokinesis initiation complex and is required to trigger centralspindlin localization as a first step in cytokinesis.

References

Aug 9, 1991·Cell·R Li, A W Murray
Nov 13, 1998·The Journal of Cell Biology·M CarmenaD M Glover
Nov 13, 1999·Nature Cell Biology·P Clute, J Pines
Jun 14, 2001·Methods : a Companion to Methods in Enzymology·A K Kenworthy
Jun 7, 2002·Molecular Cell·Jan-Michael Peters
Jul 30, 2004·Nature·Masanori MishimaMichael Glotzer
Mar 19, 2005·Science·Michael Glotzer
Sep 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wei-meng Zhao, Guowei Fang
Nov 2, 2005·The Journal of Cell Biology·Tomoya YamaguchiMasaki Inagaki
Dec 15, 2005·Journal of Cell Science·Yukako Nishimura, Shigenobu Yonemura
Dec 27, 2005·Nature Cell Biology·Hidemasa GotoMasaki Inagaki
Jun 20, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Peter KönigWolfgang Kummer
Nov 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xia YouPatrick S Daugherty
Feb 13, 2007·Current Biology : CB·Péter LénártJan-Michael Peters
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mark E BurkardPrasad V Jallepalli
Apr 10, 2007·Nature Cell Biology·Eulalie BuffinRoger E Karess
May 4, 2007·PloS One·Ian M BrennanAaron F Straight
Feb 13, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·P ThévenazM Unser
Mar 24, 2009·Nature Reviews. Molecular Cell Biology·Vincent Archambault, David M Glover

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Citations

Oct 30, 2013·Journal of Cellular Biochemistry·Bo HuangPing-Kun Zhou
Mar 12, 2016·Seminars in Cell & Developmental Biology·Masanori Mishima
Aug 14, 2012·Cytoskeleton·Clemens Cabernard
Jun 10, 2011·Communicative & Integrative Biology·Saman Ebrahimi, Stephen L Gregory
Oct 25, 2012·Spermatogenesis·Maria Grazia GiansantiRoberto Piergentili
Feb 27, 2016·Cell Cycle·Lori BorgalBernhard Schermer
Feb 8, 2020·EMBO Reports·Mario Aguilar-AragonBarry J Thompson
Feb 9, 2012·Cell Division·Bing SongXiaoqi Liu

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