Polo kinases regulate C. elegans embryonic polarity via binding to DYRK2-primed MEX-5 and MEX-6

Development
Yuichi NishiR Lin

Abstract

Polo kinases are known key regulators of cell divisions. Here we report a novel, non-cell division function for polo kinases in embryonic polarity of newly fertilized Caenorhabditis elegans embryos. We show that polo kinases, via their polo box domains, bind to and regulate the activity of two key polarity proteins, MEX-5 and MEX-6. These polo kinases are asymmetrically localized along the anteroposterior axis of newly fertilized C. elegans embryos in a pattern identical to that of MEX-5 and MEX-6. This asymmetric localization of polo kinases depends on MEX-5 and MEX-6, as well as genes regulating MEX-5 and MEX-6 asymmetry. We identify an amino acid of MEX-5, T(186), essential for polo binding and show that T(186) is important for MEX-5 function in vivo. We also show that MBK-2, a developmentally regulated DYRK2 kinase activated at meiosis II, primes T(186) for subsequent polo kinase-dependent phosphorylation. Prior phosphorylation of MEX-5 at T(186) greatly enhances phosphorylation of MEX-5 by polo kinases in vitro. Our results provide a mechanism by which MEX-5 and MEX-6 function is temporally regulated during the crucial oocyte-to-embryo transition.

References

Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F J ClayA R Dunn
Nov 5, 1997·Biochemical and Biophysical Research Communications·K E MundtE A Nigg
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·K S LeeR L Erikson
Nov 6, 1998·Nature·L Timmons, A Fire
Dec 31, 1998·Genes & Development·D M GloverA A Tavares
Jan 23, 1999·Current Opinion in Cell Biology·E A Nigg
Jun 22, 1999·DNA Sequence : the Journal of DNA Sequencing and Mapping·B OuyangD Wei
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·K S LeeR L Erikson
Jan 25, 2000·The Journal of Biological Chemistry·S HimpelW Becker
Jun 1, 2000·Cell·K Kemphues
Nov 25, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·D ChaseD K Ferris
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Young-Joo JangRaymond L Erikson
Feb 23, 2002·Trends in Cell Biology·J Wade Harper
Aug 9, 2002·Molecular and Cellular Biology·Frederic R Yarm
Aug 27, 2002·Developmental Cell·Rebecca LyczakBruce Bowerman
Jan 14, 2003·Nature Cell Biology·Mark JackmanJonathon Pines
May 10, 2003·The Journal of Biological Chemistry·Hiroyuki NakajimaEisuke Nishida
Jul 11, 2003·Developmental Cell·Tianhua ZhouRaymond L Erikson
Sep 16, 2003·Molecular and Cellular Biology·Sheng MaRaymond L Erikson
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Carrie R Cowan, Anthony A Hyman
Oct 19, 2004·Genome Research·Jean-François RualMarc Vidal
Jan 11, 2005·Oncogene·Drew M LoweryMichael B Yaffe
Jan 11, 2005·Oncogene·Frank EckerdtKlaus Strebhardt
Jan 11, 2005·Oncogene·Noriyuki TakaiIsao Miyakawa
Jan 11, 2005·Oncogene·Daniel P SeeburgMorgan Sheng

❮ Previous
Next ❯

Citations

Mar 24, 2009·Nature Reviews. Molecular Cell Biology·Vincent Archambault, David M Glover
Apr 19, 2013·Nature Reviews. Molecular Cell Biology·Carsten Hoege, Anthony A Hyman
Jul 1, 2010·BMB Reports·Matthew R Marcello, Andrew Singson
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Mohammad M RahmanOrna Cohen-Fix
Sep 21, 2011·Trends in Cell Biology·Costanza Panbianco, Monica Gotta
Oct 23, 2015·Molecular Biology of the Cell·Mohammad M RahmanOrna Cohen-Fix
Oct 15, 2010·Current Opinion in Cell Biology·Marie-Hélène VerlhacLionel Pintard
Jul 28, 2015·Experimental Cell Research·N TavernierL Pintard
Jun 4, 2015·Cell Cycle·Nicolas TavernierLionel Pintard
Mar 13, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Erik E Griffin
Aug 30, 2012·Essays in Biochemistry·Anna Noatynska, Monica Gotta
Nov 13, 2008·Current Opinion in Cell Biology·Tohru TakakiMark Petronczki
Nov 6, 2009·Current Biology : CB·Cassandra S Heighington, Edward T Kipreos
Jun 1, 2010·Cell·Daniel St Johnston, Julie Ahringer
Mar 11, 2015·The Journal of Cell Biology·Nicolas TavernierLionel Pintard
Feb 26, 2016·Molecular & Cellular Proteomics : MCP·Jia-Xuan ChenMatthias Selbach
Apr 25, 2018·Molecular Biology of the Cell·Abigail R GerholdPaul S Maddox
Dec 13, 2018·Molecular Biology of the Cell·Youjun WuErik E Griffin
Mar 1, 2019·Development·Andrew W Folkmann, Geraldine Seydoux
Mar 21, 2019·Development·Shaohe WangRebecca A Green
Mar 30, 2019·Traffic·Elisabeth A Marnik, Dustin L Updike
Jun 24, 2014·Nature Reviews. Molecular Cell Biology·Sihem ZitouniMónica Bettencourt-Dias
Jan 11, 2019·Genetics·Lionel Pintard, Bruce Bowerman
Sep 11, 2019·FEBS Letters·Saishu Yoshida, Kiyotsugu Yoshida
Oct 28, 2019·The Journal of Cell Biology·Alexandra BondazMonica Gotta
Nov 5, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sergi ArandaSusana de la Luna
Aug 25, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alicia G GubiedaJosana Rodriguez
Mar 9, 2019·Genetics·Edward T Kipreos, Sander van den Heuvel
Jul 26, 2018·Proceedings of the National Academy of Sciences of the United States of America·Youjun WuErik E Griffin
Aug 30, 2020·Nature Communications·Maikel Castellano-PozoEnrique Martinez-Perez
Oct 18, 2020·Experimental & Molecular Medicine·Chun Shik Park, H Daniel Lacorazza
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Amelia J Kim, Erik E Griffin
Apr 3, 2021·Current Biology : CB·Joachim GarbrechtAlexander Dammermann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.