Polo‑like kinase 4 promotes tumorigenesis and induces resistance to radiotherapy in glioblastoma

Oncology Reports
Jia WangHao Liu

Abstract

Glioblastoma (GBM) is one of the most malignant tumors in adults, associated with severe outcomes (median survival, <2 years). Multiple mechanisms are known to be involved in tumor recurrence and treatment resistance in GBM, however, the key regulator for GBM tumorigenesis and therapy resistance remains unclear. To clarify a novel potential functional mechanism of GBM recurrence, a wide range of experiments including in vitro molecular biological experiments and in vivo intracranial xenograft tumor models were performed in the present study. With bioinformatics analysis, polo‑like kinase 4 (PLK4) was initially identified as one of the most upregulated kinase encoding genes in GBM, which was functionally required for both in vitro cell proliferation and in vivo tumorigenesis in GBM. Clinically, an elevated PLK4 expression was observed in high grade glioma patients, which was associated with poor prognosis. In addition, PLK4 enhanced radioresistance in GBM, while PLK4 knockdown via lentivirus transfection significantly increased the radiosensitivity of GBM cells. Mechanically, PLK4 expression was markedly elevated by the exogenous overexpression of ATPase family AAA domain‑containing protein 2 (ATAD2) in GBM cells. Collectively, ...Continue Reading

Citations

May 1, 2019·International Journal of Molecular Sciences·Gianmarco PallaviciniFerdinando Di Cunto
Sep 12, 2019·Biochemical Society Transactions·Dorota Sabat-PośpiechAndrew B Fielding

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Datasets Mentioned

BETA
GSE67089

Methods Mentioned

BETA
surgical resection
xenograft
transfection
X-Ray
Assay
flow cytometry
immunoprecipitation

Software Mentioned

SPSS

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