Poly-3-hydroxybutyrate (P3HB) production by bacteria from xylose, glucose and sugarcane bagasse hydrolysate

Journal of Industrial Microbiology & Biotechnology
L F SilvaJosé Gregório Cabrera Gomez

Abstract

Fifty-five bacterial strains isolated from soil were screened for efficient poly-3-hydroxybutyrate (P3HB) biosynthesis from xylose. Three strains were also evaluated for the utilization of bagasse hydrolysate after different detoxification steps. The results showed that activated charcoal treatment is pivotal to the production of a hydrolysate easy to assimilate. Burkholderia cepacia IPT 048 and B. sacchari IPT 101 were selected for bioreactor studies, in which higher polymer contents and yields from the carbon source were observed with bagasse hydrolysate, compared with the use of analytical grade carbon sources. Polymer contents and yields, respectively, reached 62% and 0.39 g g(-1) with strain IPT 101 and 53% and 0.29 g g(-1) with strain IPT 048. A higher polymer content and yield from the carbon source was observed under P limitation, compared with N limitation, for strain IPT 101. IPT 048 showed similar performances in the presence of either growth-limiting nutrient. In high-cell-density cultures using xylose plus glucose under P limitation, both strains reached about 60 g l(-1) dry biomass, containing 60% P3HB. Polymer productivity and yield from this carbon source reached 0.47 g l(-1) h(-1) and 0.22 g g(-1), respectively.

References

Jul 1, 1990·Applied and Environmental Microbiology·B A RamsayJ A Ramsay
Jan 1, 1970·Archiv für Mikrobiologie·H G SchlegelI Krauss
May 26, 1999·Applied Microbiology and Biotechnology·D Riesenberg, R Guthke
Oct 12, 2001·International Journal of Systematic and Evolutionary Microbiology·C O BrämerA Steinbüchel
Nov 6, 2001·Applied Microbiology and Biotechnology·R V NonatoC E Rossell
Oct 1, 1990·Applied and Environmental Microbiology·J L BertrandC Chavarie
Nov 1, 1994·Applied and Environmental Microbiology·F K YoungS W May
Jan 1, 1994·World Journal of Microbiology & Biotechnology·J G PradellaA Bonomi

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Citations

Jul 16, 2011·Current Microbiology·Mateus Schreiner Garcez LopesLuiziana Ferreira da Silva
Oct 13, 2006·Bioprocess and Biosystems Engineering·Martin KollerGerhart Braunegg
Jun 9, 2006·Journal of Industrial Microbiology & Biotechnology·Thomas M KeenanStuart W Tanenbaum
Jan 15, 2008·Journal of Industrial Microbiology & Biotechnology·Yolanda González-GarcíaGerhart Braunegg
Mar 11, 2011·Journal of Molecular Microbiology and Biotechnology·M S L LopesL F Silva
Jul 26, 2014·Journal of Industrial Microbiology & Biotechnology·Mateus Schreiner Garcez LopesLuiziana Ferreira Silva
Oct 26, 2013·New Biotechnology·M Teresa CesárioM Manuela R da Fonseca
Jun 21, 2014·Journal of Applied Microbiology·E GasserH König
Jul 3, 2015·Applied Microbiology and Biotechnology·Takeharu TsugeKouhei Mizuno
Feb 23, 2013·Bioresource Technology·Javier M NaranjoCarlos A Cardona
May 18, 2016·New Biotechnology·Martin KollerGerhart Braunegg
Sep 7, 2016·Journal of Biotechnology·Neelamegam Annamalai, Nallusamy Sivakumar
Jan 20, 2018·Journal of Industrial Microbiology & Biotechnology·Linda P GuamánLuiziana Ferreira da Silva
Sep 25, 2007·Journal of Applied Microbiology·D Van-ThuocB Mattiasson
Feb 7, 2018·Applied Microbiology and Biotechnology·Su Yean OngKumar Sudesh
Nov 10, 2009·Canadian Journal of Microbiology·Mateus Schreiner Garcez LopesLuiziana Ferreira Silva
Mar 29, 2011·Advances in Dental Research·Y Chu, H Liu
Nov 28, 2013·Journal of Applied Microbiology·T T MendonçaL F Silva
Apr 23, 2020·Frontiers in Bioengineering and Biotechnology·Luz YañezFelipe Scott
Dec 22, 2019·Bioengineering·Yolanda González-GarcíaJosé Antonio Silva-Guzman
Sep 28, 2017·Bioengineering·Rodrigo Yoji Uwamori TakahashiAndré Oliveira de Souza Lima
Aug 1, 2018·Biotechnology for Biofuels·Vibhavee Sukruansuwan, Suchada Chanprateep Napathorn
Aug 27, 2017·Applied Biochemistry and Biotechnology·Jan Christoph Peinemann, Daniel Pleissner
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Maciej GuzikSławomir Wawak
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Carolina Bilia Chimello de PaulaJonas Contiero

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