Poly-adenine-Coupled LAMP Barcoding to Detect Apple Scar Skin Viroid

ACS Combinatorial Science
Se Hee LeeJi-Young Ahn

Abstract

Apple Scar Skin Viroid (ASSVd), a nonprotein coding, circular RNA pathogen is relatively difficult to detect by immunoassay. We report here a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to improve selectivity for diagnostic use in detecting ASSVd in plants. ASSVd RT-LAMP was accelerated using loop primers and was found to be highly sensitive with a detection limit of 104 copies of cDNA-ASSVd within 30 min. Real-time LAMP and melting curve analysis could differentiate between the true-positive LAMP amplicons and false-positive nonspecific primer amplification products. The optimized RT-LAMP was then followed by the addition of nonthiolated AuNP:poly-adenine (A10)-ASSVd LAMP barcodes, showing a high authentication capacity with colorimetric changes. This type of barcoding assay is a potential alternative for rapid and multiple viroid diagnosis, providing for visible sensing in the field that can be applied to viroid-free planting.

References

Apr 11, 1992·Nucleic Acids Research·G T WalkerD P Malinowski
Sep 11, 1987·Nucleic Acids Research·J Hashimoto, H Koganezawa
Oct 16, 1999·Journal of Microbiological Methods·M N WidjojoatmodjoA C Fluit
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Mar 28, 2002·Nucleic Acids Research·Zhi LiRobert L Letsinger
Jul 30, 2002·Molecular and Cellular Probes·K NagamineT Notomi
Jul 13, 2004·EMBO Reports·Myriam VincentHuimin Kong
Dec 11, 2007·Journal of Medical Microbiology·Natsu UemuraShoji Kudoh
Dec 25, 2007·Analytical and Bioanalytical Chemistry·Pedro BaptistaRicardo Franco
Jul 7, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Xuehua ZhangNobuo Maeda
Nov 28, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Xu ZhangJuewen Liu
Dec 14, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Xuehua ZhangNobuo Maeda
Apr 9, 2013·Experimental Parasitology·Rambabu SurabattulaRathnagiri Polavarapu
Oct 10, 2013·Bioconjugate Chemistry·Feng LiX Chris Le
Sep 5, 2014·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Kei NakashimaYoshihito Otsuka
Jan 16, 2015·Biosensors & Bioelectronics·Sang-Hee LeeYang-Hoon Kim
Feb 17, 2015·Journal of Virological Methods·Manabu NemotoTakashi Kondo
Feb 9, 2016·Molecular and Cellular Probes·Lorraine LillisDavid S Boyle
Nov 1, 2016·Accounts of Chemical Research·Michael G Mohsen, Eric T Kool
Feb 9, 2017·Nanoscale·Simranjeet Singh SekhonYang-Hoon Kim
May 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Myeong-Sub SongYang-Hoon Kim
May 23, 2017·Nanoscale·Simranjeet Singh SekhonYang-Hoon Kim
Jul 21, 2017·Virus Research·Jihong JiangShifang Li

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Srividhya VenkataramanKathleen Hefferon

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