Poly (ADP-ribose) polymerase mediates diabetes-induced retinal neuropathy

Mediators of Inflammation
Ghulam MohammadAhmed M Abu El-Asrar

Abstract

Retinal neuropathy is an early event in the development of diabetic retinopathy. One of the potential enzymes that are activated by oxidative stress in the diabetic retina is poly (ADP-ribose) polymerase (PARP). We investigated the effect of the PARP inhibitor 1,5-isoquinolinediol on the expression of the neurodegeneration mediators and markers in the retinas of diabetic rats. After two weeks of streptozotocin-induced diabetes, rats were treated with 1,5-isoquinolinediol (3 mg/kg/day). After 4 weeks of diabetes, the retinas were harvested and the levels of reactive oxygen species (ROS) were determined fluorometrically and the expressions of PARP, phosporylated-ERK1/2, BDNF, synaptophysin, glutamine synthetase (GS), and caspase-3 were determined by Western blot analysis. Retinal levels of ROS, PARP-1/2, phosphorylated ERK1/2, and cleaved caspase-3 were significantly increased, whereas the expressions of BDNF synaptophysin and GS were significantly decreased in the retinas of diabetic rats, compared to nondiabetic rats. Administration of 1,5-isoquinolinediol did not affect the metabolic status of the diabetic rats, but it significantly attenuated diabetes-induced upregulation of PARP, ROS, ERK1/2 phosphorylation, and cleaved casp...Continue Reading

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Citations

Apr 4, 2015·Current Eye Research·Xiaoyan JiangYan Luo
May 31, 2015·Neurobiology of Disease·Catherine A Cobb, Marsha P Cole
Apr 29, 2016·Journal of Diabetes Research·Cristina HernándezGiovanni Casini
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Apr 12, 2019·Investigative Ophthalmology & Visual Science·Krisztina KovacsBalazs Sumegi
May 16, 2020·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Nasser Al Sabaani
Nov 22, 2019·Advances in Wound Care·Jaideep BanerjeeBao-Ngoc Nguyen
Jun 29, 2021·Journal of Diabetes and Metabolic Disorders·Regina Ngozi UgbajaOluseyi Adeboye Akinloye

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Methods Mentioned

BETA
protein assay

Software Mentioned

GeneTools

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