Poly I:C enhances cycloheximide-induced apoptosis of tumor cells through TLR3 pathway

BMC Cancer
Qun JiangZhigang Tian

Abstract

Toll-like receptor 3 (TLR3) is a critical component of the innate immune response to dsRNA viruses, which was considered to be mainly expressed in immune cells and some endothelial cells. In this study, we investigated the expression and proapoptotic activity of TLR3 in human and murine tumor cell lines. RT-PCR and FACS analysis were used to detect expression of TLR3 in various human and murine tumor cell lines. All tumor cell lines were cultured with poly I:C, CHX, or both for 12 h, 24 h, 72 h, and then the cell viability was analyzed with CellTiter 96(R) AQueous One Solution, the apoptosis was measured by FACS with Annexin V and PI staining. Production of Type I IFN in poly I:C/CHX mediated apoptosis were detected through western blotting. TLR3 antibodies and IFN-beta antibodies were used in Blockade and Neutralization Assay. We show that TLR3 are widely expressed on human and murine tumor cell lines, and activation of TLR3 signaling in cancerous cells by poly I:C made Hela cells (human cervical cancer) and MCA38 cells (murine colon cancer) become dose-dependently sensitive to protein synthesis inhibitor cycloheximide (CHX)-induced apoptosis. Blockade of TLR3 recognition with anti-TLR3 antibody greatly attenuated the proapopt...Continue Reading

References

Apr 1, 1995·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·A L KhanO Eremin
May 15, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N TanakaT Taniguchi
Apr 28, 1999·Trends in Microbiology·H Everett, G McFadden
Oct 19, 2001·Genes and Immunity·M MiettinenS Matikainen
Jul 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mamta Chawla-SarkarErnest C Borden
May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·M Chawla-SarkarE C Borden
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·William J KaiserMargaret K Offermann
Apr 30, 2004·Nature Immunology·Gregory M Barton, Ruslan Medzhitov
May 18, 2004·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Shin-Hun JuangWen K Yang
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Aug 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Klaus RuckdeschelJürgen Heesemann
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·William J Kaiser, Margaret K Offermann
Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bruno SalaunToufic Renno
Apr 28, 2006·Journal of Gastroenterology and Hepatology·Akiko SatoSumio Watanabe
Jan 24, 2007·Journal of Cellular Biochemistry·Elina KhvalevskyHilla Giladi

❮ Previous
Next ❯

Citations

Feb 26, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Qiang ZhouHao Cheng
Jul 8, 2011·Clinical & Experimental Metastasis·Tanja Matijevic, Jasminka Pavelic
Mar 9, 2012·Targeted Oncology·Nadège GoutagnyChristophe Caux
Nov 10, 2012·Endocrine·Myriem BoufraqechElectron Kebebew
Sep 4, 2012·Archives of Pharmacal Research·Shaherin BasithSangdun Choi
Oct 16, 2012·Cancer Biotherapy & Radiopharmaceuticals·Xiangzhong ZhaoChengfang Yao
Mar 12, 2009·Drugs·Johnathan D EbbenJohn S Kuo
May 14, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Daye ChengYiran Ma
Jan 18, 2011·Critical Reviews in Oncology/hematology·Jahangir AbdiFrank Redegeld
Jul 1, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yingjun Lv, Endong Bao
Nov 18, 2008·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Uyen M LeZhengrong Cui
Jan 25, 2011·Experimental Dermatology·Ning YuLeihong Xiang
Jun 9, 2014·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Zhifeng HeQingang Hu
May 10, 2012·The International Journal of Biochemistry & Cell Biology·Qingqiong LuoFuxiang Chen
Nov 11, 2011·Oral Oncology·Hui-Ching ChuangJiin-Haur Chuang
Feb 26, 2013·The Journal of Allergy and Clinical Immunology·Rebeca Pérez de DiegoJasminka Godovac-Zimmermann
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shishir Kumar GuptaA P Sahoo
Dec 8, 2017·Yonsei Medical Journal·Ying ChenMei Zhang
May 15, 2012·Journal of Leukocyte Biology·Hsin-Chun HuangKuender D Yang
Apr 21, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Giovanni ForteRosalinda Sorrentino
Aug 15, 2018·International Journal of Molecular Sciences·Hideki NakamuraAtsushi Kawakami
Jun 8, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takanori NumataKazuyoshi Kuwano

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry
Assay
FACS

Software Mentioned

WINMDI2

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis