PMID: 7202031Jan 1, 1981

Poly I:C/poly-L-lysine: potent inducer of interferons in primates

Journal of Interferon Research
G P LampsonM R Hilleman

Abstract

A soluble complex of poly I:C and poly-L-lysine (poly I:C/poly-L-lysine) has been prepared that induces high titers of circulating interferon in monkeys. By limiting the molar ratio of lysine to nucleotide to 0.5, a complex was formed that was soluble up to 2.0 mg poly I:C/ml of phosphate-buffered saline. Complexes of poly I:C with poly-L-lysine of various molecular weights, and in a constant ratio (0.5) of lysine to nucleotide, were evaluated for capacity to induce serum interferon in grivet monkeys. Substantial enhancement (10- to 100-fold) of the capacity of poly I:C to induce interferon in grivet monkeys was observed using poly I:C complexed with poly-L-lysines of molecular weight 10(4) daltons or greater. The poly I:C/poly-L-lysine as an effective inducer of interferon in grivet monkeys, rhesus monkeys, chimpanzees and marmosets. A high interferon titer (greater than 100 units/ml blood) was maintained in grivet monkeys by repeated daily administration of the complex. No long-term hyporesponsiveness was noted following repeat inductions over a period of months. The serum interferon produced in monkeys in response to poly I:C/poly-L-lysine resembled human leukocyte interferon in its biological characteristics.

References

Oct 1, 1975·The Journal of Infectious Diseases·H B LevyJ Rice
Oct 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·G P LampsonM R Hilleman
Apr 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·A K FieldM R Hilleman
Sep 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·A K FieldM R Hilleman
Nov 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·M M NemesM R Hilleman
Feb 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·J J NordlundH B Levy
Jan 1, 1966·Journal of Molecular Biology·M TsuboiP O Ts'o
Sep 1, 1980·Proceedings of the Society for Experimental Biology and Medicine·A K FieldA A Tytell

Citations

May 14, 2005·Antiviral Chemistry & Chemotherapy·Michael Markosian, Robyn M Hyde
Jan 1, 1984·Journal of Interferon Research·C M DeWitt, A K Field
Nov 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Joyce PoastS S Baron
Jan 1, 1984·Journal of Interferon Research·H MachidaY Watanabe
Jan 1, 1983·Journal of Interferon Research·M E Davies, A K Field
Jan 1, 1984·Journal of Interferon Research·E N GranadosJ Bello
Apr 27, 2018·Cancer Immunology, Immunotherapy : CII·Hussein SultanEsteban Celis

Related Concepts

Cercopithecus tantalus
Drug Combinations
Interferon Inducers
Polypeptides
Poly I-C
Poly-(Alpha-L-Lysine)

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