Poly(3-Hydroxybutyrate) Production with High Productivity and High Polymer Content by a Fed-Batch Culture of Alcaligenes latus under Nitrogen Limitation.

Applied and Environmental Microbiology
F Wang, S Y Lee

Abstract

Alcaligenes latus has been known to produce poly(3-hydroxybutyrate) (PHB) in a growth-associated manner even under nutrient-sufficient conditions. However, the PHB content obtained by fed-batch culture was always low, at ca. 50%, which makes the recovery process inefficient. In this study, the effect of applying nitrogen limitation on the production of PHB by A. latus was examined. In flask and batch cultures, the PHB synthesis rate could be increased considerably by applying nitrogen limitation. The PHB content could be increased to 87% by applying nitrogen limitation in batch culture, which was considerably higher than that typically obtainable (50%) under nitrogen-sufficient conditions. In fed-batch culture, cells were first cultured by the DO-stat feeding strategy without applying nitrogen limitation. Nitrogen limitation was applied at a cell concentration of 76 g (dry cell weight)/liter, and the sucrose concentration was maintained within 5 to 20 g/liter. After 8 h of nitrogen limitation, the cell concentration, PHB concentration, and PHB content reached 111.7 g (dry cell weight)/liter, 98.7 g/liter, and 88%, respectively, resulting in a productivity of 4.94 g of PHB/liter/h. The highest PHB productivity, 5.13 g/liter/h, w...Continue Reading

References

Jan 1, 1997·Nature Biotechnology·S Y Lee
Jan 5, 1996·Biotechnology and Bioengineering·S Y Lee

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Citations

Sep 13, 2019·International Journal of Biological Macromolecules·Doan Van ThuocKumar Sudesh
Jul 23, 2009·Chemical Society Reviews·Guo-Qiang Chen
Dec 22, 2007·Applied Microbiology and Biotechnology·Jorge QuillaguamánRajni Hatti-Kaul
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Nov 5, 2016·Applied Biochemistry and Biotechnology·Li-Jung HsiaoSi-Yu Li
May 29, 1999·Applied and Environmental Microbiology·S Y LeeJ Y Song

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