Poly(A)- and poly(U)-specific RNA 3' tail shortening by E. coli ribonuclease E

Nature
H HuangS N Cohen

Abstract

Ribonuclease (RNase) E is an extensively studied enzyme from Escherichia coli whose site-specific endoribonuclease activity on single-stranded RNA has a central role in the processing of ribosomal RNA, the degradation of messenger RNA and the control of replication of ColE1-type plasmids. Here we report a previously undetected activity of RNase E: the ability to shorten 3' poly(A)- and poly(U)-homopolymer tails on RNA molecules. This activity, which leaves a 6-nucleotide adenylate or a 1-nucleotide uridylate remnant on primary transcripts, resides in the amino-terminal region of RNase E and does not require other protein cofactors. Addition of a 3'-terminal phosphate group prevents both removal of the poly(A) tail and endonucleolytic cleavage within primary transcripts, but has no effect on the cleavage of transcripts with tails that have already been truncated. The ability of RNase E to shorten poly(A) tails, together with the effect of tail length on endonucleolytic cleavage within primary transcripts, suggests a mechanism by which RNase E may exercise overall control over RNA decay.

References

Oct 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C P EhretsmannH M Krisch
Mar 24, 1995·Cell·S N Cohen
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F XuS N Cohen
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·E HajnsdorfP Régnier
Nov 1, 1994·Molecular Microbiology·B PyC F Higgins
Jan 12, 1996·The Journal of Biological Chemistry·G A Coburn, G A Mackie
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·A MiczakS Lin-Chao
Nov 1, 1996·Microbiology·N Sarkar
Mar 1, 1997·Molecular Microbiology·S N Cohen, K J McDowall

❮ Previous
Next ❯

Citations

May 14, 1999·Trends in Biochemical Sciences·R HayesW Gruissem
Apr 4, 2002·Molecular Microbiology·Chaitanya JainJoel G Belasco
Apr 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Oleg N MurashkoSue Lin-Chao
Nov 20, 2001·Nucleic Acids Research·S JägerG Klug
May 9, 2001·Nucleic Acids Research·A P WalshK J McDowall
Aug 10, 2002·Journal of Bacteriology·Sidney R Kushner
Mar 14, 2007·Molecular and Cellular Biology·Olivia S RisslandChris J Norbury
Jan 11, 2000·Molecular and Cellular Biology·M T McManusS L Hajduk
Dec 14, 2006·Microbial Cell Factories·Christian Kemmer, Peter Neubauer
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y Feng, S N Cohen
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·E Hajnsdorf, P Régnier
Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yanan FengStanley N Cohen
Aug 12, 2005·Journal of Molecular Recognition : JMR·Vladimir N UverskyA Keith Dunker
Feb 23, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Régnier, C M Arraiano
Feb 24, 2011·Wiley Interdisciplinary Reviews. RNA·Bijoy K Mohanty, Sidney R Kushner
Nov 26, 2010·The Plant Journal : for Cell and Molecular Biology·Michael WalterRalph Bock
May 16, 2007·Physical Review Letters·Yeonee SeolAvraham Halperin
Apr 20, 2001·The Journal of Biological Chemistry·K Lee, S N Cohen
Mar 30, 2001·The Journal of Biological Chemistry·R Aphasizhev, L Simpson
Jan 5, 2007·The Biochemical Journal·Mirijam-Elisabeth ZellerVladimir R Kaberdin
Jun 17, 1999·FEMS Microbiology Reviews·R Rauhut, G Klug
Oct 10, 2012·Molecular Microbiology·Patricia B LodatoJames B Kaper
Jun 17, 1999·FEMS Microbiology Reviews·A W Nicholson
Dec 18, 2012·Nature Reviews. Microbiology·George A Mackie
Jun 27, 2020·Research in Microbiology·Huaduo YanWenli Chen
Jul 3, 2020·Journal of Theoretical Biology·Thibault A EtienneDelphine Ropers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.