Poly(A)-driven and poly(A)-assisted termination: two different modes of poly(A)-dependent transcription termination.

Molecular and Cellular Biology
G YeungH G Martinson

Abstract

We mapped the elements that mediate termination of transcription downstream of the chicken betaH- and betaA-globin gene poly(A) sites. We found no unique element and no segment of 3'-flanking DNA to be significantly more effective than any other. When we replaced the native 3'-flanking DNA with bacterial DNA, it too supported transcription termination. Termination in the bacterial DNA depended on a functional poly(A) signal, which apparently compelled termination to occur in the downstream DNA with little regard for its sequence. We also studied premature termination by poorly processive polymerases close to the promoter. The rate of premature termination varied for different DNA sequences. However, the efficiencies of poly(A)-driven termination and promoter-proximal premature termination varied similarly on different DNAs, suggesting that poly(A)-driven termination functions by returning the transcription complex to a form which resembles a prior state of low processivity. The poly(A)-driven termination described here differs dramatically from the poly(A)-assisted termination previously described for the simian virus 40 (SV40) early transcription unit. In the SV40 early transcription unit, essentially no termination occurs dow...Continue Reading

References

Jul 20, 1991·Journal of Molecular Biology·D J VandenberghR C Hardison
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M N Szentirmay, M Sawadogo
Oct 1, 1990·Molecular and Cellular Biology·R TischN Hozumi
Jan 1, 1990·Methods in Enzymology·R J Kaufman
Jan 1, 1985·Molecular and Cellular Biology·M L RohrbaughR C Hardison
Mar 1, 1989·Trends in Biochemical Sciences·N J Proudfoot
Apr 1, 1986·Molecular and Cellular Biology·K SatoK Agarwal
Dec 1, 1988·Molecular and Cellular Biology·T M Pribyl, H G Martinson
Jul 1, 1986·Journal of Virology·S IwamotoJ E Darnell
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M Sawadogo, R G Roeder
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A Maderious, S Chen-Kiang
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C M GormanB H Howard
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Feb 10, 1995·The Journal of Biological Chemistry·S M Berget
Apr 1, 1993·Genes & Development·K M Wassarman, J A Steitz
Jun 1, 1993·Molecular and Cellular Biology·T O'Brien, J T Lis
Jan 15, 1996·Genes & Development·H LouS M Berget
May 1, 1996·Molecular and Cellular Biology·J BlauD Bentley
Jul 1, 1996·Molecular and Cellular Biology·K Y YankulovD L Bentley
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·H OkamotoB M Peterlin

❮ Previous
Next ❯

Citations

Oct 5, 2001·Molecular and Cellular Biology·D P TranH G Martinson
Jun 19, 2007·Nature Structural & Molecular Biology·Anita NagHarold G Martinson
Jul 9, 2002·Molecular and Cellular Biology·Martha L PetersonFrankie Davis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.