PMID: 6159918Dec 1, 1980Paper

Polyacrylamide gel electrophoresis of amniotic fluid cholinesterases: a good prenatal test for neural tube defects

British Journal of Obstetrics and Gynaecology
M J Seller, K J Cole

Abstract

The qualitative assay of the cholinesterases (ChE) in amniotic fluid on polyacrylamide gel gave a single major band (cholinesterase) in all samples from normal pregnancies, and two major bands (cholinesterase and acetylcholinesterase) in all cases from fetuses with open neural tube defects. Five fluids which were true false positive on alpha-fetoprotein (AFP) assay (elevated AFP in a clear fluid but normal fetus) had a single band, and two fluids which were false negative on AFP testing (normal AFP but spina bifida fetus) had two bands. The second 'diagnostic' ChE band sometimes occurred, together with other extra bands, in some fluids which were very severely contaminated by maternal or fetal blood, but in four of six fluids from normal fetuses where fetal blood staining was sufficient to cause the AFP to be elevated, there was only one ChE band. It is suggested that the qualitative assay of ChE should be performed in addition to AFP in the prenatal diagnosis of neural tube defects.

References

Nov 18, 1975·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·I W Chubb, A D Smith
Jun 1, 1980·British Journal of Obstetrics and Gynaecology·M J SellerP E Polani
Dec 28, 1964·Annals of the New York Academy of Sciences·J T CLARKE

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Citations

Aug 4, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·A F Smith
Feb 26, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·K J Cole, M J Seller
Dec 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·J R Bonham, J R Atack
Nov 30, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·A JeziorowskaB Kałuzewski
Jun 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·A G RasmussenB Norgaard-Pedersen
Feb 14, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·L D JayanthiA S Balasubramanian
Feb 21, 1981·Lancet·R B Payne
Feb 21, 1981·Lancet·M J Seller, A C Berry
Jan 1, 1992·International Journal of Obstetric Anesthesia·P N BakerJ G Williamson
Feb 1, 1982·British Journal of Obstetrics and Gynaecology·A P ReadR Harris
Oct 1, 1982·British Journal of Obstetrics and Gynaecology·R D BarlowC H Rodeck
Dec 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·J HangaardB Nørgaard-Pedersen
Dec 1, 1981·Journal of Medical Genetics·M J Seller

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