Poly(acrylic acid)-poly(ethylene glycol) layers on positively charged surface coatings: molecular structure, protein resistance, and application to single protein deposition

Langmuir : the ACS Journal of Surfaces and Colloids
Andrea SeehuberReiner Dahint

Abstract

A new copolymer (PAA-PEG2000) has been designed, consisting of a negatively charged poly(acrylic acid) (PAA) backbone to which poly(ethylene glycol) (PEG) side chains with a molecular weight of about 2 kDa were grafted in a molecular ratio of 3:10. It readily adsorbs to positively charged surfaces and may be considered to be the anionic counterpart of PEG-grafted poly(l-lysine) (PLL-PEG), which was first described by Kenausis et al. and is widely used to render negatively charged surfaces protein-resistant. The synthesis of PAA-PEG2000 can be carried out in aqueous solution at room temperature and does not require any sophisticated techniques such as handling in an inert gas atmosphere. Using ellipsometry and infrared reflection absorption spectroscopy (IRRAS), the film structure has been carefully analyzed for copolymer adsorption onto three different positively charged surfaces, namely, thin layers of poly(allylamine) (PAH), poly(ethyleneimine) (PEI) and (3-aminopropyl)triethoxysilane (APTES). Besides the film thickness, the conformation of the PEG chains and their orientation with respect to the surface normal appear to be important parameters for the protein resistance of the films. Although PAA-PEG2000 adsorbed to PAH and ...Continue Reading

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Citations

Dec 12, 2012·Biomacromolecules·M F DelcroixC C Dupont-Gillain
May 21, 2013·The Journal of Physical Chemistry. B·A Seehuber, R Dahint
Oct 15, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Tobias PeisskerCarmen Bartic
Jan 13, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaoying ZhuG Julius Vancso
Sep 11, 2014·Macromolecular Bioscience·Peter J F RöttgermannJoachim O Rädler

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