PMID: 9348Apr 1, 1976

Poly(adenosine diphosphate ribose) is covalently linked to nuclear proteins by two types of bonds

Hoppe-Seyler's Zeitschrift für physiologische Chemie
P Adamietz, H Hilz

Abstract

(ADP-ribose)n residues formed by short-term incubation of adult rat liver and Ehrlich carcinoma nuclei with labeled NAD were analyzed by Cs2SO4/guanidinium chloride/urea density gradient centrifugation. Comparison with samples in which the protein had been completely digested revealed that most, or probably all, acid-insoluble (ADP-ribose)n chains are covalently bound to nuclear proteins, as is true for the short, acid-soluble (ADP-ribose)n chains. Complete release of (ADP-ribose)n chains is effected by dilute alkali. In contrast, NH2OH liberated only part of the long and the short (ADP-ribose)n residues from the protein conjugates, indicating two types of bonds, both alkali-labile, but only one susceptible to neutral hydroxylamine. Both types of bonds were equally distributed among acid-soluble and acid-insoluble (ADP-ribose)n chains. -Stability of the (ADP-ribose)n protein conjugates during isolation is only guaranteed at pH values below 7.

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·K UedaO Hayaishi
Jan 1, 1969·Cold Spring Harbor Symposia on Quantitative Biology·Y NishizukaO Hayaishi
Aug 6, 1973·Biochemical and Biophysical Research Communications·R A ColyerW R Kidwell
Sep 5, 1973·Biochemical and Biophysical Research Communications·J A Smith, L A Stocken

Citations

Jan 1, 1978·Advances in Enzyme Regulation·H HilzK Wielckens
Jan 1, 1982·Advances in Enzyme Regulation·K WielckensH Hilz
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Y OhashiO Hayaishi
May 14, 2008·Molecular and Cellular Biology·Stuart L RultenKeith W Caldecott
Jan 8, 2013·Epigenetics & Chromatin·Ingrid KassnerMichael O Hottiger
Jan 1, 1986·CRC Critical Reviews in Biochemistry·R S WuW M Bonner
Oct 3, 1977·European Journal of Biochemistry·D RickwoodW J Whish
Jan 2, 1978·European Journal of Biochemistry·R BredehorstH Hilz
Sep 30, 2014·Proteomics·Christina A Vivelo, Anthony K L Leung
Jun 20, 2015·Molecular Cell·Casey M DanielsAnthony K L Leung
Apr 28, 1978·Biochemical and Biophysical Research Communications·P AdamietzH Hilz
Jul 16, 1981·Biochemical and Biophysical Research Communications·P AdamietzH Hilz
Oct 1, 1985·Bioscience Reports·A Johnstone, R McNerney
Jun 13, 2014·Journal of Proteome Research·Casey M DanielsAnthony K L Leung
Apr 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·R BredehorstG Siebert
Jan 1, 1979·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K WielckensH Hilz
Jun 13, 2019·Biological Chemistry·Jürgen LassakWolfram Volkwein

Related Concepts

Carcinoma, Ehrlich Tumor
Cell Nucleus
Centrifugation, Density Gradient
Hydrogen-Ion Concentration
Liver
NADH
Nucleoside Diphosphate Sugars
Peptide Hydrolases
Poly Adenosine Diphosphate Ribose
Sodium Hydroxide

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