Dec 1, 1975

Polyadenylated RNA from Vicia faba meristematic root cells. Localization and size estimation of the poly (A) segment

European Journal of Biochemistry
R EsnaultR B van Huystee


After incubating root apices from two-day-old bean seedlings with [3H] adenine the RNA was extracted from whole cells or polysomes, and the poly (A) sequences were isolated by nuclease digestion followed by poly(U)-Sepharose chromatography. The alterations of the RNA molecules due to the various treatments were monitored by sucrose density gradients. It was found that sequential extraction first at pH 7.6 then at pH 9.0 did not result in a separation between RNA poor in poly(A) sequences and poly(A)-rich RNA. Furthermore chromatography analysis of hydrolysates from nuclease-resistant RNA extracted either at pH 7.6 or pH 9.0 revealed that AMP constituted nearly 95% of the bases and that the poly(A) sequences, about 200 bases, were located at the 3' terminus of the polyadenylated RNA. No size difference was found for the poly(A) segment between the pH-7.6-extracted RNA and that extracted at pH 9.0.

Mentioned in this Paper

Establishment and Maintenance of Localization
Centrifugation, Density Gradient
Set of Polysomal Ribosomes
Adenine Polynucleotides
RNA, Polyadenylated
Chromatography, Agarose

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