PMID: 9445028Jan 28, 1998Paper

Polyadenylation of influenza virus mRNA transcribed in vitro from model virion RNA templates: requirement for 5' conserved sequences

Journal of Virology
D C PritloveGeorge G Brownlee

Abstract

Here we report the development of two independent assays which demonstrate for the first time that exogenous model RNA templates based on influenza virus virion RNA (vRNA) are transcribed in vitro to produce polyadenylated mRNA. We investigated the activities of mutated templates with known polymerase binding properties to test our model that polyadenylation occurs when a polymerase complex, which is bound to conserved 5' sequences of vRNA, prevents read-through of the U track at which polyadenylation subsequently occurs by reiterative copying. Mutated templates with perturbed polymerase binding sites (i.e., a deletion mutant lacking the first 4 5' residues and a U-->A point mutant at the third residue) initiated transcription in the in vitro assay but failed to produce polyadenylated transcripts, whereas an A-->U point mutant at the fourth residue, which retained polymerase binding properties similar to those of the wild type, produced polyadenylated transcripts. Our results show that nucleotides within the conserved 5' sequence are required for polyadenylation and support the hypothesis that polymerase binding to 5' sequences of the template is required for mRNA synthesis.

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Citations

Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·I H GregerN Proudfoot
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·S H BaeB S Choi
Jun 4, 2010·The Journal of General Virology·Nicole C RobbErvin Fodor
Sep 20, 2012·Molecular BioSystems·Henna KimByong-Seok Choi
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ayae HondaAkira Ishihama
Dec 15, 2006·Journal of Virology·F T Vreede, G G Brownlee
Oct 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Philip J KranzuschSean P J Whelan
Mar 22, 2002·The Journal of General Virology·Agustín Portela, Paul Digard
Jun 20, 2002·Journal of Virology·George G Brownlee, Jane L Sharps
Mar 22, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K HaraT Toyoda
May 15, 2002·Microbiology and Immunology·Yasushi OhtsuTetsuya Toyoda
Dec 16, 2019·Molecular Neurobiology·Maria Czarnek, Joanna Bereta
Oct 27, 2017·Viruses·Alex HarwigBen Berkhout
Jul 11, 1998·Journal of Virology·E FodorA García-Sastre
May 11, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jesse D Pyle, Sean P J Whelan
Aug 29, 2018·Veterinary Sciences·Jessica BenkarounAndrew S Lang
Jul 11, 2021·Communications Biology·Masahiro NakanoTakeshi Noda
Nov 26, 2009·Biochemical and Biophysical Research Communications·Shijian ZhangTetsuya Toyoda

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