Polyadenylation of RNA in vitro in isolated chromatin and nuclei.

European Journal of Biochemistry
D I De Pomerai, P H Butterworth

Abstract

Poly(A) is added post-transcriptionally to RNA transcribed in vitro by endogenous form B DNA-dependent RNA polymerase bound to the template in isolated nuclei or chromatin. It is also added to processed fragments of the products from alpha-amanitin-resistant RNA polymerases A and/or C. The poly(A) segments are of similar size to those found in nuclear RNA pulse-labelled in vivo and are added onto the 3' terminus of RNA chains (whether pre-existing, completed during the incubation in vitro or created by fragmentation of larger RNA transcripts). That poly(A) addition is not directly mediated by any of the nuclear DNA-dependent RNA polymerases is shown by the differential sensitivities of RNA and poly(A) syntheses to increasing ionic strength and transcriptional inhibitors such as the exotoxin from Bacillus thuringiensis.

References

Feb 21, 1975·European Journal of Biochemistry·T J Beebee, P H Butterworth
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L A PhillipsV W Hollis
Jun 25, 1974·Journal of Molecular Biology·J GorskiJ B Lingrel
Sep 23, 1974·Biochemical and Biophysical Research Communications·J Niessing, C E Sekeris
Sep 9, 1974·Biochemical and Biophysical Research Communications·K M Rose, S T Jacob
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R SheldonJ Kates
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A JacobsonH Lodish
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R Weinmann, R G Roeder
Sep 28, 1973·Science·J E DarnellG R Molloy
Jun 28, 1972·Journal of Molecular Biology·R H Reeder, R G Roeder
Oct 25, 1973·Journal of Molecular Biology·R H Reeder
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J H CramerH O Halvorson
Jul 2, 1973·European Journal of Biochemistry·I Grummt, R Lindigkeit
Jun 27, 1974·Biochimica Et Biophysica Acta·S J Kaufman, K W Gross
Aug 1, 1974·European Journal of Biochemistry·D I de PomeraiP H Butterworth
Jun 28, 1972·Journal of Molecular Biology·M Adesnik, J E Darnell
Jan 31, 1972·Biochimica Et Biophysica Acta·G P GeorgievE R Avakyan
Nov 11, 1971·European Journal of Biochemistry·P H ButterworthC J Chesterton
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M EdmondsH Nakazato
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·L PhilipsonJ E Darnell

❮ Previous
Next ❯

Citations

Jan 1, 1982·Preparative Biochemistry·S Nadin-Davis, V A Mezl
Jan 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K P Schäfer
Sep 20, 1977·Biochimica Et Biophysica Acta·K M RoseS T Jacob
May 1, 1977·Journal of Virology·B Vennström, L Philipson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.