Poly(ADP-ribose) effectively competes with DNA for histone H4 binding

Biochemical and Biophysical Research Communications
G Sauermann, J Wesierska-Gadek

Abstract

The effect of poly(ADP-ribose) on DNA-histone H4 interaction was studied using a nitrocellulose filter binding assay. Poly-(ADP-ribose) was found to form poly(ADP-ribose)-histone H4 complexes at physiological salt concentrations. The homopolymer effectively competed with DNA for histone H4 binding. Poly(ADP-ribose) was also capable of displacing DNA from preformed DNA-histone H4 complexes. Our hypothesis is that poly(ADP-ribose), locally and transiently formed at the site of DNA damage, causes dissociation of DNA from the nucleosome particle or nucleosome unfolding.

References

Jan 1, 1976·Reviews of Physiology, Biochemistry and Pharmacology·H Hilz, P Stone
Aug 15, 1985·The Biochemical Journal·J C Gaal, C K Pearson
Jan 1, 1985·Annual Review of Biochemistry·K Ueda, O Hayaishi
May 24, 1974·Science·R D Kornberg, J O Thomas
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K McEnteeI R Lehman
Jan 1, 1982·Progress in Nucleic Acid Research and Molecular Biology·P MandelC Niedergang
Nov 1, 1982·European Journal of Biochemistry·M WongM Smulson
Oct 11, 1984·Nature·T J RichmondA Klug
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G G PoirierP Mandel
Apr 15, 1983·Biochemical and Biophysical Research Communications·K HayashiT Sugimura
May 25, 1980·Journal of Molecular Biology·V V ShickA D Mirzabekov

❮ Previous
Next ❯

Citations

May 26, 1993·Molecular and Cellular Biochemistry·D LautierG G Poirier
Oct 27, 1992·Biochimica Et Biophysica Acta·H ThomassinG G Poirier
Dec 27, 2012·Molecular Aspects of Medicine·Jana KrietschJean-Philippe Gagné
Jan 8, 2013·Molecular Aspects of Medicine·Alexander Bürkle, László Virág
Feb 21, 2009·PLoS Genetics·Elena KotovaAlexei V Tulin
May 2, 1988·European Journal of Biochemistry·J Wesierska-Gadek, G Sauermann
Apr 5, 2020·Cancers·Juan Manuel MartíFrancisco Javier Oliver

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.