Poly(ADP-ribose) glycohydrolase silencing protects against H2O2-induced cell death

The Biochemical Journal
Christian BlennMaria Malanga

Abstract

PAR [poly(ADP-ribose)] is a structural and regulatory component of multiprotein complexes in eukaryotic cells. PAR catabolism is accelerated under genotoxic stress conditions and this is largely attributable to the activity of a PARG (PAR glycohydrolase). To overcome the early embryonic lethality of parg-knockout mice and gain more insights into the biological functions of PARG, we used an RNA interference approach. We found that as little as 10% of PARG protein is sufficient to ensure basic cellular functions: PARG-silenced murine and human cells proliferated normally through several subculturing rounds and they were able to repair DNA damage induced by sublethal doses of H2O2. However, cell survival following treatment with higher concentrations of H2O2 (0.05-1 mM) was increased. In fact, PARG-silenced cells were more resistant than their wild-type counterparts to oxidant-induced apoptosis while exhibiting delayed PAR degradation and transient accumulation of ADP-ribose polymers longer than 15-mers at early stages of drug treatment. No difference was observed in response to the DNA alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine, suggesting a specific involvement of PARG in the cellular response to oxidative DNA damage.

References

Jun 1, 1992·Analytical Biochemistry·T Boulikas
Jan 31, 1972·Biochemical and Biophysical Research Communications·K UedaO Hayaishi
Jan 1, 1997·Free Radical Biology & Medicine·A M GardnerA Lichtenstein
Oct 4, 2000·The Journal of Biological Chemistry·J M PleschkeF R Althaus
Oct 29, 2000·The Journal of Biological Chemistry·E B AffarG G Poirier
Jul 20, 2001·Experimental Cell Research·L DavidovicG G Poirier
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·W YingR A Swanson
Mar 12, 2003·Biochimie·Laura RossiA Ivana Scovassi
May 3, 2003·The EMBO Journal·Josiane Ménissier de MurciaGilbert de Murcia
May 16, 2003·Molecular and Cellular Biology·Satoshi OkanoAkira Yasui
Jun 28, 2003·Experimental Hematology·Véronique J BouchardGuy G Poirier
Jul 23, 2003·Molecular and Cellular Biochemistry·Silvia Di MeglioPiera Quesada
Jul 25, 2003·Biochemical and Biophysical Research Communications·Sayaka OhashiMasanao Miwa
Sep 23, 2003·Nucleic Acids Research·Sherif F El-KhamisyKeith W Caldecott
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shuji HanaiMasanao Miwa
May 4, 2004·Trends in Pharmacological Sciences·Suk Jin HongValina L Dawson
Jul 27, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jean-Christophe AméGilbert de Murcia
Aug 18, 2004·Biochemical Pharmacology·A Ivana Scovassi, Marc Diederich
Dec 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·David W KohTed M Dawson
Jan 29, 2005·Journal of the American Society of Nephrology : JASN·Nimesh S A PatelChristoph Thiemermann
Mar 26, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Salvatore CuzzocreaZhao-Qi Wang
Apr 20, 2005·Oncogene·Sandra L Harris, Arnold J Levine
May 25, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Grazia Graziani, Csaba Szabó
Jun 17, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Maria Malanga, Felix R Althaus
Jun 25, 2005·Molecular Pharmacology·Katalin ErdèlyiLászló Virag
Aug 25, 2005·The Biochemical Journal·Jean-Philippe GagnéGuy G Poirier
Sep 6, 2005·Genes & Development·Mi Young KimW Lee Kraus
Nov 10, 2005·The Journal of Biological Chemistry·Shunya OkaJoel Moss
Feb 8, 2006·Biochimica Et Biophysica Acta·Jean-François HainceGuy G Poirier

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Citations

Sep 30, 2010·Cellular and Molecular Life Sciences : CMLS·C BlennFelix R Althaus
Apr 13, 2010·Pathology Oncology Research : POR·Nilufer Jasmine Selimah FauzeeYa-lan Wang
Jan 10, 2012·ACS Chemical Biology·Kristin E FinchPaul J Hergenrother
Jul 3, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katalin ErdélyiLászló Virág
Mar 23, 2011·Molecular Cancer Research : MCR·Yongxian Zhuang, W Keith Miskimins
Dec 8, 2009·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalPremendu P Mathur
Mar 26, 2009·PloS One·David M BurnsRaymond A Swanson
Jun 19, 2013·PloS One·Salomé C Vilchez LarreaSilvia H Fernández Villamil
Jan 28, 2014·PloS One·W Keith MiskiminsJoon Young Choi
May 24, 2014·Cell Death & Disease·A SahabogluF Paquet-Durand
Aug 1, 2014·BMC Complementary and Alternative Medicine·David O AdeyemiStephen O Adewole
Aug 10, 2013·Translational Stroke Research·Paco S HersonPatricia D Hurn
Dec 3, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ikram El ZaouiAlicia Torriglia
Jul 5, 2016·Cancer Cell International·Aurélia NollValérie Schreiber
Nov 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Masato MashimoJoel Moss
May 20, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Frances M MunozTerrence J Monks
Jan 1, 2012·Genes·Christian BlennFelix R Althaus
Feb 24, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Christian BlennFelix R Althaus
Oct 16, 2016·F1000Research·Dominic I JamesDonald J Ogilvie
Apr 30, 2008·Current Opinion in Anaesthesiology·Domokos Gerö, Csaba Szabó
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Weihai Ying
Jun 10, 2008·The Journal of Biological Chemistry·Tiziana GuastafierroPaola Caiafa
May 23, 2008·The Journal of Biological Chemistry·Maria MalangaFelix R Althaus
Apr 2, 2014·British Journal of Pharmacology·Amos A FatokunTed M Dawson
Dec 17, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Fumiaki UchiumiSei-ichi Tanuma
Jan 21, 2019·Neurochemical Research·Nina Klimova, Tibor Kristian
Feb 1, 2011·FEBS Letters·Adriana FuriaGiuseppe D'Alessio

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