Poly(ADP-ribose) polymerase inhibits DNA synthesis initiation in the absence of NAD

Biochemical and Biophysical Research Communications
T NoboriD A Carson

Abstract

Poly(ADP-ribose) polymerase (ADPRP) is a nuclear enzyme that transfers ADP-ribose from NAD+ to diverse nuclear proteins. Previously, the function of ADPRP was considered to relate exclusively to its catalytic activity. However, recent experiments have shown that ADPRP is actually an abundant DNA-binding protein, and that the potential catalytic activity of the enzyme is more than 100-fold greater than the measured rates of NAD+ turnover in intact cells. To better understand the role of ADPRP, we have used highly purified ADPRP and a monospecific autoantibody to examine the effects of ADPRP on in vitro DNA synthesis in the presence or absence of NAD+ substrate. The data show that DNA synthesis initiation is blocked by ADPRP and that auto-poly (ADP-ribosyl)ation reverses the process by diminishing the DNA binding capacity of the protein. These results suggest that ADPRP actually is a structural DNA binding protein, whose catalytic activity serves to modulate its interaction with DNA.

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·K UedaO Hayaishi
Jan 1, 1977·Annual Review of Biochemistry·O Hayaishi, K Ueda
Oct 24, 1977·Biochemical and Biophysical Research Communications·K YoshiharaH Ohgushi
Sep 1, 1987·The Journal of Clinical Investigation·H YamanakaD A Carson
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S H LeeJ Hurwitz
Jan 1, 1985·Annual Review of Biochemistry·K Ueda, O Hayaishi
Feb 28, 1970·Journal of Molecular Biology·A D RiggsS Bourgeois
Mar 16, 1983·Biochemical and Biophysical Research Communications·A NishioE M Uyeki

❮ Previous
Next ❯

Citations

May 26, 1993·Molecular and Cellular Biochemistry·D LautierG G Poirier
Oct 31, 1991·Biochemical and Biophysical Research Communications·K G BukiE Kun
Mar 5, 2010·Nature Reviews. Cancer·Michèle RouleauGuy G Poirier
May 1, 1993·Biological Reviews of the Cambridge Philosophical Society·T Alderson
Jun 17, 2016·Molecular & Cellular Oncology·Antonella SistiguIlio Vitale
May 20, 2015·Scientific Reports·Katherine S YangRalph Weissleder

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.