Poly(ADP-ribosylation) is present in murine sciatic nerve fibers and is altered in a Charcot-Marie-Tooth-1E neurodegenerative model

PeerJ
Laura I Lafon HughesAlejandra E Kun González

Abstract

Poly-ADP-ribose (PAR) is a polymer synthesized by poly-ADP-ribose polymerases (PARPs) as a postranslational protein modification and catabolized mainly by poly-ADP-ribose glycohydrolase (PARG). In spite of the existence of cytoplasmic PARPs and PARG, research has been focused on nuclear PARPs and PAR, demonstrating roles in the maintenance of chromatin architecture and the participation in DNA damage responses and transcriptional regulation. We have recently detected non-nuclear PAR structurally and functionally associated to the E-cadherin rich zonula adherens and the actin cytoskeleton of VERO epithelial cells. Myelinating Schwann cells (SC) are stabilized by E-cadherin rich autotypic adherens junctions (AJ). We wondered whether PAR would map to these regions. Besides, we have demonstrated an altered microfilament pattern in peripheral nerves of Trembler-J (Tr-J) model of CMT1-E. We hypothesized that cytoplasmic PAR would accompany such modified F-actin pattern. Wild-type (WT) and Tr-J mice sciatic nerves cryosections were subjected to immunohistofluorescence with anti-PAR antibodies (including antibody validation), F-actin detection with a phalloidin probe and DAPI/DNA counterstaining. Confocal image stacks were subjected to...Continue Reading

References

Oct 27, 1992·Biochimica Et Biophysica Acta·H ThomassinG G Poirier
Aug 25, 1990·Nucleic Acids Research·H ThomassinG G Poirier
Jul 1, 1987·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·L Ménard, G G Poirier
Apr 1, 1997·Journal of Anatomy·A M RobertsonP K Thomas
Mar 10, 1999·Brain : a Journal of Neurology·S LoveG K Wilcock
Sep 12, 2002·Pharmacological Reviews·László Virág, Csaba Szabó
Feb 26, 2003·Cell·Mirna Perez-MorenoElaine Fuchs
Apr 2, 2003·Cell Biology Education·Bruce Alberts, UNKNOWN National Academies
Sep 24, 2004·Physiological Reviews·M CereijidoL Shoshani
Jan 26, 2005·Acta Neuropathologica·José C VisBerry Kremer
Feb 11, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jérôme J Devaux, Steven S Scherer
Apr 1, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicolas TricaudUrs Rutishauser
May 4, 2005·Cellular and Molecular Life Sciences : CMLS·M MasutaniT Sugimura
Nov 2, 2005·Nucleic Acids Research·Constanze BaranekMichael Wegner
Mar 27, 2007·Molecular and Cellular Neurosciences·Claire Perrin-TricaudNicolas Tricaud
May 19, 2007·Experimental Cell Research·Ralph G MeyerMyron K Jacobson
Apr 12, 2008·Mutation Research·Laura Lafon-HughesWilner Martínez-López
Sep 23, 2008·Glia·M Laura FeltriJoão B Relvas
Jun 12, 2009·Genes & Development·Stephen P J FancyDavid H Rowitch
Jan 21, 2010·Journal of Cell Science·Xiao PengKris A DeMali
May 12, 2010·Cold Spring Harbor Perspectives in Biology·Wenxiang Meng, Masatoshi Takeichi
Jun 15, 2010·Nucleosides, Nucleotides & Nucleic Acids·B CerboniS Sestini
Jun 16, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Courtney M KarnerThomas J Carroll
Jun 28, 2011·Nature Neuroscience·Stephen P J FancyDavid H Rowitch
Jul 28, 2011·Nature Neuroscience·Patrizia Casaccia
Sep 17, 2011·Bioarchitecture·Hwan Tae Park, M Laura Feltri
Mar 21, 2012·Molecular Neurobiology·Joanna B StrosznajderRobert P Strosznajder
Jun 7, 2012·Nucleic Acids Research·Jean-Philippe GagnéGuy G Poirier
Sep 4, 2012·Neurobiology of Disease·Jochen KinterNicole Schaeren-Wiemers
Dec 12, 2012·Molecular Neurobiology·Jun LiJiasong Guo
Jan 4, 2013·Journal of the Peripheral Nervous System : JPNS·James L Salzer

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Citations

Jan 30, 2019·International Journal of Molecular Sciences·Michelle SchackeLaura Lafon-Hughes

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Methods Mentioned

BETA
PARylation
immunoprecipitation
confocal microscopy
dissection

Software Mentioned

Excel
ImageJ
Fluoview
Image J

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