PMID: 2493263Mar 1, 1989Paper

Poly(ADP-ribosylation) of nuclear proteins in rat testis correlates with active spermatogenesis

Biochimica Et Biophysica Acta
P QuesadaR Jones

Abstract

Poly(ADP-ribosylation) of nuclear proteins has been investigated in rat testis under different experimental conditions to determine whether it is associated with somatic or germinal cells. Isolated, intact nuclei were incubated with [14C]NAD and extracted sequentially with 5% HClO4 and 0.25 M HCl, and labelled soluble proteins were analysed by reverse-phase high-performance liquid chromatography and acetic acid-urea polyacrylamide gel electrophoresis (pH 2.9). Results show that in normal adult testis a major acceptor protein for poly(ADP-ribose) in HClO4 extracts is the tissue-specific histone, H1t. Core histones and three proteins (alpha, beta and gamma) with low mobility on acetic acid-urea gels were the major acceptors identified in HCl extracts. Poly(ADP-ribosylation) of all the aforementioned proteins is very low in isolated intact nuclei of testis from 8-day-old animals (only spermatogonia present in seminiferous tubules), increases significantly by 16-day (pachytene spermatocytes appear) and reaches adult proportions by 32 days (condensing spermatids present). In the nuclei from cryptorchid testes, poly(ADP-ribosylation) of nuclear proteins resembles 8-day-old testis. It is concluded that (a) poly(ADP-ribosylation) of nu...Continue Reading

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Citations

Apr 20, 2005·Chromosoma·Mirella L Meyer-FiccaRalph G Meyer
Jan 1, 1995·Biochimie·F R AlthausB Zweifel
Sep 16, 2000·Insect Biochemistry and Molecular Biology·M DenegriA Ivana Scovassi
Oct 1, 2010·Biology of Reproduction·Mirella L Meyer-FiccaRalph G Meyer
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Paul O HassaMichael O Hottiger
Jul 26, 2013·Spermatogenesis·Ciler Celik-Ozenci, Arda Tasatargil
Jul 31, 1990·Biochemical and Biophysical Research Communications·P QuesadaB Farina
Dec 2, 1999·Journal of Cellular Biochemistry·M R Faraone-MennellaB Farina
Dec 17, 2008·Molecular and Cellular Biochemistry·Maria Rosaria Faraone-MennellaBenedetta Farina
Oct 6, 2000·Archives of Biochemistry and Biophysics·L AtorinoP Quesada
Oct 10, 1998·Biochimica Et Biophysica Acta·M MalangaP Quesada

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