PMID: 3766944Aug 1, 1986Paper

Poly(ADP-ribosyl)ation studies in situ

Analytical Biochemistry
D M Duhl, L S Hnilica

Abstract

We have studied the poly(ADP-ribosyl)ation of nuclear proteins in situ by examining the incorporation of [3H]NAD-derived ADP-ribose into polymers. We have devised a way to deliver [3H]NAD to cells growing in vitro, and we have determined the kinetics of uptake and incorporation into nuclear proteins using this delivery system. Incorporation into the histone fraction, known acceptors of poly(ADP-ribose), was examined and shown to be sensitive to the poly(ADP-ribose) polymerase inhibitor 3-aminobenzamide. Polyacrylamide gel electrophoresis of 3H-labeled proteins revealed radioactivity associated with known poly(ADP-ribose)-accepting proteins such as poly(ADP-ribose) polymerase and histones. These results were confirmed when we immunoreacted gel-separated proteins with anti-(ADP-ribose) generated in our laboratory.

References

Jan 1, 1977·Annual Review of Biochemistry·O Hayaishi, K Ueda
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·G Poste, D Papahadjopoulos
Sep 23, 1974·Biochimica Et Biophysica Acta·D PapahadjopoulosE Mayhew
Jan 1, 1968·Analytical Biochemistry·P V Tishler, C J Epstein
Aug 31, 1981·Biochemical and Biophysical Research Communications·B W DurkaczS Shall
Nov 30, 1981·Biochemical and Biophysical Research Communications·J Jongstra-BilenP Mandel
Mar 1, 1982·Canadian Journal of Biochemistry·R J AubinG G Poirier
Jan 1, 1982·Progress in Nucleic Acid Research and Molecular Biology·P MandelC Niedergang
Sep 1, 1983·Bioscience Reports·G T Williams, A P Johnstone
Dec 1, 1983·Carcinogenesis·T Sugimura, M Miwa
Apr 1, 1984·The Biochemical Journal·H M WallaceC K Pearson
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Y OhashiO Hayaishi
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R ReevesS C Chung
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M WongM Smulson
Oct 3, 1983·European Journal of Biochemistry·M TavassoliS Shall
Sep 30, 1983·Biochemical and Biophysical Research Communications·M K Song, K W Adolph
Sep 1, 1980·Canadian Journal of Biochemistry·A LarocheG G Poirier
Jun 1, 1980·Canadian Journal of Biochemistry·G G Poirier, P Savard
May 20, 1983·Biochimica Et Biophysica Acta·C S Surowy, N A Berger
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K IkaiO Hayaishi
Jul 1, 1982·The Biochemical Journal·M R MennellaR Jones
Jul 30, 1981·Biochemical and Biophysical Research Communications·M KawaichiO Hayaishi
Jul 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·B F ERLANGER, S M BEISER

❮ Previous
Next ❯

Citations

May 1, 1989·Biological Reviews of the Cambridge Philosophical Society·T Alderson
May 2, 1989·Biochemistry·G Krupitza, P Cerutti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.