Polyamine- and Amino Acid-Related Metabolism: The Roles of Arginine and Ornithine are Associated with the Embryogenic Potential

Plant & Cell Physiology
Leandro Francisco de OliveiraEny Iochevet Segal Floh

Abstract

The mechanisms that control polyamine (PA) metabolism in plant cell lines with different embryogenic potential are not well understood. This study involved the use of two Araucaria angustifolia cell lines, one of which was defined as being blocked, in that the cells were incapable of developing somatic embryos, and the other as being responsive, as the cells could generate somatic embryos. Cellular PA metabolism was modulated by using 5 mM arginine (Arg) or ornithine (Orn) at two time points during cell growth. Two days after subculturing with Arg, an increase in citrulline (Cit) content was observed, followed by a higher expression of genes related to PA catabolism in the responsive cell line; whereas, in the blocked cell line, we only observed an accumulation of PAs. After 14 d, metabolism was directed towards putrescine accumulation in both cell lines. Exogenous Arg and Orn not only caused a change in cellular contents of PAs, but also altered the abundance of a broader spectrum of amino acids. Specifically, Cit was the predominant amino acid. We also noted changes in the expression of genes related to PA biosynthesis and catabolism. These results indicate that Arg and Orn act as regulators of both biosynthetic and catabolic...Continue Reading

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Citations

May 17, 2018·Journal of Experimental Botany·Alex CostaIldiko Szabo
Mar 14, 2021·Plant Physiology and Biochemistry : PPB·Neidiquele M SilveiraEduardo C Machado
May 8, 2021·Free Radical Research·Ana Luiza Dorigan de Matos FurlanettoSilvia Maria Suter Correia Cadena
May 25, 2021·Saudi Journal of Biological Sciences·Riti Thapar KapoorParvaiz Ahmad

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