Polyamine depletion inhibits the differentiation of L6 myoblast cells

Biochemical and Biophysical Research Communications
B G ErwinA E Pegg

Abstract

Exposure to alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor of ornithine decarboxylase, inhibited the insulin induced differentiation of L6 myoblast cells. Differentiation was assessed by measuring creatine kinase activity and by determining the percentage of nuclei in myotubes. The levels of putrescine and spermidine increased in stimulated cultures prior to their differentiation and these increases were blocked by alpha-difluoromethylornithine. Provision of exogenous putrescine was able to reverse the inhibitory effect of the drug. The anti-differentiative effect is observed only if alpha-difluoromethylornithine is added within twenty-four hours of insulin stimulation. In the experimental protocol used, alpha-difluoromethylornithine was added as the cultures approached confluence and had no effect on their ultimate DNA content. Therefore, the effect of alpha-difluoromethylornithine on myoblast differentiation is not secondary to an effect on cellular proliferation. These results indicate that polyamines may be involved in the mediation of muscle cell differentiation.

References

Sep 1, 1971·Developmental Biology·I R Konigsberg
May 1, 1971·Developmental Biology·A ShainbergD Yaffe
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Yaffe
Aug 1, 1981·Developmental Biology·D Z Ewton, J R Florini
Jan 1, 1981·Differentiation; Research in Biological Diversity·O Heby
Sep 1, 1982·Biochemical Pharmacology·B G Erwin, A E Pegg
Jan 15, 1981·Analytical Biochemistry·B G ErwinJ R Florini

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Citations

Nov 1, 1988·Cytotechnology·N MoustaïdA Quignard-Boulangé
Dec 1, 1984·In Vitro·J R FloriniB M Vertel
Aug 3, 2011·Amino Acids·Ikumi IshiiAkira Shirahata
Jul 26, 1985·Biochimica Et Biophysica Acta·S BeninatiF Autuori
Aug 15, 2000·Molecular and Cellular Endocrinology·M E FerioliO Pinotti
Mar 1, 1989·Environmental Health Perspectives·G CameronB T Mossman
Feb 1, 1986·Journal of Cellular Physiology·C Multhauf, J Lough
Sep 1, 1984·Journal of Cellular Physiology·D Z EwtonJ R Florini
Apr 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T HayashiT Suda
Feb 15, 1987·International Journal of Cancer. Journal International Du Cancer·S KubotaF Takaku
Jul 1, 1989·International Journal of Radiation Biology·A BeccioliniM Attanasio
Mar 1, 1984·The American Journal of Physiology·B G ErwinA E Pegg

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