PMID: 2494298Apr 1, 1989Paper

Polyamines and Ca2+ mediate hyperosmolal opening of the blood-brain barrier: in vitro studies in isolated rat cerebral capillaries

Journal of Neurochemistry
H KoenigJ J Trout

Abstract

We recently presented evidence that the reversible opening of the blood-brain barrier (BBB) by the infusion of 1.6 M mannitol into the rat internal carotid artery is mediated by a rapid stimulation of ornithine decarboxylase (ODC) activity and putrescine synthesis in cerebral capillaries. We have now investigated this hypothesis further, using isolated rat cerebral capillaries as an in vitro model of the BBB. The ODC activity of cerebral capillary preparations was enriched up to 15-fold over that of the cerebral homogenate. Hyperosmolal mannitol in physiological buffer evoked a rapid (less than 15 s), concentration- and time-dependent increase in capillary ODC activity and an accumulation of putrescine and spermidine which was blocked by the specific ODC inhibitor, alpha-difluoromethylornithine (DFMO, 10 mM). Mannitol (1 M), as well as 2 M urea, evoked a two- to fivefold increase in the temperature-sensitive influx of 45Ca2+ and uptake of horseradish peroxidase (HRP) and 2-deoxy-D-[1-3H]glucose (DG), but not alpha-[1-14C]aminoisobutyrate, during a 2-min incubation. DFMO (10 mM) abolished 1 M mannitol-mediated stimulation of 45Ca2+ influx and uptake of HRP and DG, whereas 1 mM putrescine replenished capillary polyamines and reve...Continue Reading

References

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Citations

Dec 17, 2002·Bioelectromagnetics·K-A Hossmann, D M Hermann
Jan 1, 1991·Neuroradiology·J Greenwood
Jun 1, 1992·Molecular and Chemical Neuropathology·W Paschen
Dec 1, 1993·Neurochemistry International·F Joó
Jul 16, 2013·Progress in Neurobiology·Marijke De BockLuc Leybaert
Dec 12, 1997·Journal of the Neurological Sciences·D M Hermann, K A Hossmann
Jan 1, 1996·Journal of Basic and Clinical Physiology and Pharmacology·L GlantzE Shohami
Mar 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·W PaschenG Röhn
Jun 1, 1992·Journal of Clinical Pharmacology·W KoenigV Hombach
Oct 9, 2009·Electromagnetic Biology and Medicine·Bahriye Sirav, Nesrin Seyhan
Mar 4, 2015·Cold Spring Harbor Protocols·Elke DecrockLuc Leybaert

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