Abstract
In the present study we analysed polyamine metabolism in Arabidopsis thaliana (ecotype Columbia) flowers and stalks collected from plants germinated and grown under increasing salt-stress conditions (0-75 mM NaCl). The expression level of the different isoforms of polyamine biosynthetic enzymes was analysed by reverse transcriptase-polymerase chain reaction (RT-PCR). Spermidine synthase enzyme activity determined both in supernatant and pellet fractions, together with RT-PCR results, led us to hypothesize a different intracellular compartmentation of the isoforms of these enzymes. Free and conjugated polyamines (perchloric acid-soluble and -insoluble) were measured. Free spermidine was the most abundant polyamine and its levels, such as those of free spermine, increased with salt concentration, supporting the hypothesis for a specific role of those polyamines in the response and tolerance to salt stress of Arabidopsis thaliana flowers.
References
Feb 15, 1996·The Biochemical Journal·A J MichaelD Burtin
Aug 1, 1996·Plant Physiology·M B Watson, R L Malmberg
Sep 11, 1999·FEBS Letters·S Soyka, A G Heyer
Aug 16, 2000·The EMBO Journal·Y HanzawaY Komeda
Jan 5, 2001·The Biochemical Journal·M FranceschettiA J Michael
May 17, 2001·Amino Acids·N Bagni, A Tassoni
Jan 1, 1990·Photochemistry and Photobiology·N Hamasaki, A W Galston
Sep 29, 2001·The Plant Journal : for Cell and Molecular Biology·C HanfreyA J Michael
Sep 11, 2002·FEBS Letters·Yoshie HanzawaTaku Takahashi
Oct 9, 2002·The Plant Cell·Mireia PanicotAntonio F Tiburcio
Nov 13, 2002·Plant Physiology·Miguel A Perez-AmadorJuan Carbonell
Dec 20, 2003·Biochemical and Biophysical Research Communications·Kaoru UranoKazuo Shinozaki
Mar 23, 2004·Physiologia Plantarum·Cristina BortolottiTeresa Altabella
Apr 17, 2004·Physiologia Plantarum·Vasuki Kasinathan, Astrid Wingler
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Teresa CapellPaul Christou
Jun 25, 2004·Plant & Cell Physiology·Yoshihisa KasukabeShoji Tachibana
Feb 14, 2006·Journal of Plant Physiology·Alexis Ndayiragije, Stanley Lutts
Aug 2, 2006·Journal of Plant Physiology·Peter A Roussos, Constantine A Pontikis
Nov 14, 2006·Plant Physiology and Biochemistry : PPB·N BagniA Tassoni
Dec 5, 2006·FEBS Letters·Koji YamaguchiTomonobu Kusano
Mar 14, 2007·Journal of Plant Research·Tomonobu KusanoYoshihiro Takahashi
Jun 15, 2007·FEBS Letters·Jürgen M KnottManfred Sumper
Citations
Aug 24, 2011·Amino Acids·Lin ShaoSubhash C Minocha
Nov 26, 2009·Functional & Integrative Genomics·Tilahun AbebeRoger P Wise
Sep 18, 2012·Applied Biochemistry and Biotechnology·Apiradee PothipongsaAran Incharoensakdi
Mar 5, 2013·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Mandar SenguptaSarmistha Sen Raychaudhuri
Mar 9, 2012·Proteome Science·Rui FanDeyue Yu
Nov 7, 2009·Science·Eric A Hanushek
Jul 30, 2011·Science·Wolfgang Lutz, Samir KC
Dec 15, 2015·Journal of Plant Research·Jing DuJin Sun
Mar 9, 2010·Plant Physiology and Biochemistry : PPB·Rubén AlcázarTeresa Altabella
Feb 9, 2010·Plant Physiology and Biochemistry : PPB·Yukie NakaYoshihiro Takahashi
Jun 30, 2010·The Plant Journal : for Cell and Molecular Biology·Savithri NambeesanAvtar K Handa
Aug 5, 2010·Physiologia Plantarum·Sikander Pal ChoudharyMukesh Kanwar
Apr 9, 2014·GM Crops & Food·Malabika Roy PathakShabir H Wani
Sep 28, 2016·Frontiers in Plant Science·Kamala GuptaBhaskar Gupta
Jan 23, 2016·Plant, Cell & Environment·Xavier ZarzaRubén Alcázar
Sep 1, 2018·Journal of Experimental Botany·Marianne DelporteDavid Gagneul
Apr 1, 2011·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Pranjal Hazarika, Manchikatla Venkat Rajam
Feb 6, 2020·Plant, Cell & Environment·Jing CuiGuillaume Tcherkez
Dec 8, 2017·Plant, Cell & Environment·Chunliu ZhuoShaoyun Lu
Oct 12, 2017·Frontiers in Plant Science·Jianhao LuoShaoyun Lu
Feb 13, 2021·Plants·Chrystalla AntoniouVasileios Fotopoulos
Jun 1, 2021·Frontiers in Plant Science·Naga Charan KonakallaAndreas Voloudakis